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. 2016 Oct 12;7(46):76036–76046. doi: 10.18632/oncotarget.12582

Figure 2. SOX17 directly interacts with MAML3.

Figure 2

HEC-1B cells overexpressing SOX17 were seeded on 96-well plates. IF analysis showed that MAML3 (red) co-localized with SOX17 (green) in HEC-1B cell nuclei A. HEK-293 cells were transfected with SOX17 and MAML3 cDNA. Lysates were prepared and SOX17 antibody was used for Co-IP assays, followed by western blot (WB) analysis using MAML3 antibodies B. HEC-1B cells were transfected with SOX17 cDNA and analyzed via Co-IP and WB C. Different fragments of SOX17 and MAML3 D. were expressed in HEK-293T cells and evaluated by WB using HA and c-Myc antibodies.