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. 2016 Oct 12;7(46):76036–76046. doi: 10.18632/oncotarget.12582

Figure 5. SOX17 suppressed EC cell proliferation in vitro.

Figure 5

HEC-1B cells were transfected with either SOX17 cDNA or shRNA plasmids. SOX17 expression was analyzed by western blot A1. Cell viability was assessed by MTT assay daily for 4 days A2. Colony formation assays were performed on HEC-1B cells for 2 weeks. (*P<0.05) A3. HEC-1B cells were co-transfected with SOX17/MAML3 shRNA or cDNA plasmids, and analyzed by western blot B1. Cell viability was assessed by MTT assay daily for 4 days B2. Colony formation assays were performed for 2 weeks (*P<0.05) B3. HEC-1B cells were transfected with SOX17 cDNA/MAML3 shRNA or SOX17 shRNA/MAML3 cDNA plasmids and analyzed via western bolt C1. Cell viability was assessed by MTT assay daily for 4 days C2. Colony formation assays were performed for 2 weeks (*P<0.05) C3. Ishikawa cells were analyzed following the same treatments (*P<0.05) D1.-F3.