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. 1983 Oct;80(19):6032–6035. doi: 10.1073/pnas.80.19.6032

Induction of immune responses in vivo with small numbers of veiled (dendritic) cells.

S C Knight, J Mertin, A Stackpoole, J Clark
PMCID: PMC534354  PMID: 6604279

Abstract

The role of dendritic or veiled cells (VC) from lymph nodes or spleens of rats and mice in initiating immune responses in vivo has been investigated. Host-versus-graft responses were induced by injection of VC from spleens of (C57BL/10 X CBA) F1 mice into the footpads of parental strain (CBA) animals and measured by the increase in the weight of the draining popliteal lymph nodes. The potency of VC to induce the responses was 100-fold greater than that of unseparated spleen cells. The in vivo effect of VC was not limited to this direct allogeneic stimulation because autologous VC could also be used in the induction of an experimental autoimmune disease. In these studies, experimental allergic encephalomyelitis was produced in Lewis rats by injection of guinea pig brain and spinal cord material emulsified in Freund's complete adjuvant. Small numbers of VC from spleens or lymph nodes of rats showing clinical signs of experimental allergic encephalomyelitis induced a mild form of the disease when injected intravenously into normal Lewis rats. Thus, VC carrying antigen, either as an integral part of their surface membrane or acquired during exposure to antigenic substances, appeared to be very potent agents for the induction of immune responses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fujinami R. S., Paterson P. Y. Cellular transfer of experimental allergic encephalomyelitis employing suckling and adult Lewis rats. J Clin Lab Immunol. 1981 Jul;6(1):75–79. [PubMed] [Google Scholar]
  2. Holda J. H., Silberg D., Swanborg R. H. Autoimmune effector cells. IV. Induction of experimental allergic encephalomyelitis in Lewis rats without adjuvant. J Immunol. 1983 Feb;130(2):732–734. [PubMed] [Google Scholar]
  3. Klinkert W. E., LaBadie J. H., O'Brien J. P., Beyer C. F., Bowers W. E. Rat dendritic cells function as accessory cells and control the production of a soluble factor required for mitogenic responses of T lymphocytes. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5414–5418. doi: 10.1073/pnas.77.9.5414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Knight S. C., Balfour B. M., O'Brien J., Buttifant L., Sumerska T., Clarke J. Role of veiled cells in lymphocyte activation. Eur J Immunol. 1982 Dec;12(12):1057–1060. doi: 10.1002/eji.1830121214. [DOI] [PubMed] [Google Scholar]
  5. Knight S. C., O'Brien J. A., Farrant J. Injury to human granulocytes at low temperatures. Cryobiology. 1980 Jun;17(3):273–281. doi: 10.1016/0011-2240(80)90034-6. [DOI] [PubMed] [Google Scholar]
  6. Lechler R. I., Batchelor J. R. Restoration of immunogenicity to passenger cell-depleted kidney allografts by the addition of donor strain dendritic cells. J Exp Med. 1982 Jan 1;155(1):31–41. doi: 10.1084/jem.155.1.31. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mason D. W., Pugh C. W., Webb M. The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies. Immunology. 1981 Sep;44(1):75–87. [PMC free article] [PubMed] [Google Scholar]
  8. Mertin J., Stackpoole A. Anti-PGE antibodies inhibit in vivo development of cell-mediated immunity. Nature. 1981 Dec 3;294(5840):456–458. doi: 10.1038/294456a0. [DOI] [PubMed] [Google Scholar]
  9. Mertin J., Stackpoole A. The spleen is required for the suppression of experimental allergic encephalomyelitis by prostaglandin precursors. Clin Exp Immunol. 1979 Jun;36(3):449–455. [PMC free article] [PubMed] [Google Scholar]
  10. Nussenzweig M. C., Steinman R. M. Contribution of dendritic cells to stimulation of the murine syngeneic mixed leukocyte reaction. J Exp Med. 1980 May 1;151(5):1196–1212. doi: 10.1084/jem.151.5.1196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nussenzweig M. C., Steinman R. M., Gutchinov B., Cohn Z. A. Dendritic cells are accessory cells for the development of anti-trinitrophenyl cytotoxic T lymphocytes. J Exp Med. 1980 Oct 1;152(4):1070–1084. doi: 10.1084/jem.152.4.1070. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. PATERSON P. Y. Transfer of allergic encephalomyelitis in rats by means of lymph node cells. J Exp Med. 1960 Jan 1;111:119–136. doi: 10.1084/jem.111.1.119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Panitch H. S., McFarlin D. E. Experimental allergic encephalomyelitis: enhancement of cell-mediated transfer by concanavalin A. J Immunol. 1977 Sep;119(3):1134–1137. [PubMed] [Google Scholar]
  14. Phillips M. L., Parker J. W., Frelinger J. A., O'Brien R. L. Characterization of responding cells in oxidative mitogen stimulation. II. Identification of an Ia-bearing adherent accessory cell. J Immunol. 1980 Jun;124(6):2700–2707. [PubMed] [Google Scholar]
  15. Richert J. R., Driscoll B. F., Kies M. W., Alvord E. C., Jr Adoptive transfer of experimental allergic encephalomyelitis: incubation of rat spleen cells with specific antigen. J Immunol. 1979 Feb;122(2):494–496. [PubMed] [Google Scholar]
  16. Steinman R. M., Gutchinov B., Witmer M. D., Nussenzweig M. C. Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J Exp Med. 1983 Feb 1;157(2):613–627. doi: 10.1084/jem.157.2.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Steinman R. M., Witmer M. D. Lymphoid dendritic cells are potent stimulators of the primary mixed leukocyte reaction in mice. Proc Natl Acad Sci U S A. 1978 Oct;75(10):5132–5136. doi: 10.1073/pnas.75.10.5132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sunshine G. H., Katz D. R., Feldmann M. Dendritic cells induce T cell proliferation to synthetic antigens under Ir gene control. J Exp Med. 1980 Dec 1;152(6):1817–1822. doi: 10.1084/jem.152.6.1817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Van Voorhis W. C., Hair L. S., Steinman R. M., Kaplan G. Human dendritic cells. Enrichment and characterization from peripheral blood. J Exp Med. 1982 Apr 1;155(4):1172–1187. doi: 10.1084/jem.155.4.1172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. de Vere Tyndall A., Knight S. C., Edwards A. J., Clarke J. B. Veiled (dendritic) cells in synovial fluid. Lancet. 1983 Feb 26;1(8322):472–473. doi: 10.1016/s0140-6736(83)91468-x. [DOI] [PubMed] [Google Scholar]

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