A novel method of identifying genetic mutations using an electrochemical DNA array. Wakai Junko, Takagi Atsuko, Nakayama Masato, Miya Takahito, Miyahara Takatoshi, Iwanaga Tsuyoshi, Takenaka Shigeori, Ikeda Yasuyuki, Amano Masahiko. Nucleic Acids Res. 2004;32:e141. doi: 10.1093/nar/gnh141.
The authors would like to apologize for the omission of Tomohiro Urata, from TUM Gene, Inc., 3-1 Kazusa-Koito Kimitsu, Chiba 292-1149, Japan, from the author list of this paper.
The authors would also like to apologize for two incorrect probes listed in Table 1. The 10th and 14th probes should be A261T-M and W382X-M instead of A261T-W and W382X-W, respectively. The complete corrected Table 1 is given below.
Table 1. Primers (a and b) and probe (c) sequences.
| (a) First PCR primer | |
| Ex3-FP | CTGTGCCAATGGGTTTCCA |
| Ex3-RP | CACTGTTTTGGACACATAAGTCTC |
| Ex5-FP | GAAATTTACAAATCTGTGTTCCTGCT |
| Ex5-RP | CATTGGGTCAATAAGGGTTAAGGA |
| Ex6-FP | AGACATGCCAAATGAAACACTCT |
| Ex6-RP | ACTCCTTGGTTTCCTTATTTACAACA |
| Ex7-FP | TTCATAAAGATTGATCAACATGTTCGA |
| Ex7-RP | ACTGGTGCCATGATGACCG |
| Ex8-FP | GAGAGCTGATCTCTATAACTAACCA |
| Ex8-RP | CTCTGATCTTCTGAATGGCGA |
| (b) Second PCR primer | |
| Y61X-CP | TAGGTGGGTATTTTAAGAAAGCTTGT |
| Y61X-SP | GAGAGTTGGGTGCCTCTCTCTTGTACAGGGCGGCCACAAG |
| V200A-CP | GTAGACGTCTTACACACATTCACCAGAGGG |
| V200A-SP | TGGGCATGTTGACATCCTGGCTGAAAAGTACCTCCATTCGGG |
| A221-del-CP | CAGTTGGGCATGTTGACATTTACCCG |
| A221-del-SP | CTATCCGCGTGATTCTTCTAAATAATATTTACCTCCAAGTCCTCTCTCTGC |
| R243C-CP | GCACCTGTAGGCCTTACTTGGATTTTCT |
| R243C-SP | CTCGTGGGAGCACACCCAGATGTGGACCAGCTAGTGAA |
| A261T-CP | CCCACGAGCGCTCCATTCATCTCT |
| A261T-SP | CCTACAGGTGCAGTAGAGCCCTTTCTCAAAGGCTTCCTTGG |
| A334T-CP | CCTCCCCAACAGTCTTCCATTACCAAGTAAAG |
| A334T-SP | CCTTTGAGATTTCTCTGGGATGTTCTCACTCTCGGCCACGGTGCCAT |
| W382X-CP | AGACCTACTCCTTCCTAATTTACACAGAGGTAG |
| W382X-SP | AAGAGTGATTCATACTTTCTGCTCCACCAGTCTGACCAGC |
| (c) Probe | |
| Y61X-W probe | AAAGCTTGTGTCATCATCTTCAGGTAACAGGAATGTAT |
| Y61X-M probe | AAAGCTTGTGTCATCATCTTCAGGTAACAGGAATGTAA |
| V200A-W probe | GGGTCCCCTGGTCGAAGCATTGGAATCCAGAAACCAGT |
| V200A-M probe | GGGTCCCCTGGTCGAAGCATTGGAATCCAGAAACCAGC |
| A221-del-W probe | TGGAGGTACTTTTCAGCCAGGATGTAACATTGGAGAAG |
| A221-del-M probe | ATGGAGGTACTTTTCAGCCAGGATGTAACATTGGAGAA |
| R243C-W probe | TCATTCAACAGAGAGTCGATGAAGAGATGAATGGAGCG |
| R243C-M probe | TCATTCAACAGAGAGTCGATGAAGAGATGAATGGAGCA |
| A261T-W probe | CATCGACTCTCTGTTGAATGAAGAAAATCCAAGTAAGG |
| A261T-M probe | CATCGACTCTCTGTTGAATGAAGAAAATCCAAGTAAGA |
| A334T-W probe | TTTTTCTGGGACTGAGAGTGAAACCCATACCAATCAGG |
| A334T-M probe | TTTTTCTGGGACTGAGAGTGAAACCCATACCAATCAGA |
| W382X-W probe | TAGATATTGGAGAACTACTCATGTTGAAGCTCAAATGG |
| W382X-M probe | TAGATATTGGAGAACTACTCATGTTGAAGCTCAAATGA |
In (a) FP denotes ‘forward primer’ and RP denotes ‘reverse primer’. In (b) CP and SP indicate ‘counter primer’ and ‘special primer’, respectively. The underlined nucleotides in the SP are the tag sequences essential to make a stem structure in a self-loop formation (cf. Figure 3D). In (c) W probe and M probe indicate ‘wild type probe’ and ‘mutant type probe’, respectively.