Table 1.
Peptide | Abz-peptidyl-EDDnp | kcat/KM (106M−1s−1) |
---|---|---|
PAR 1 | Abz-TLDPR↓SFLLQ-EDDnp* | 2.8 |
PAR 3 | Abz-TLPIKTFR↓GQ-EDDnp | 3.6 |
PAR 4 | Abz-LPAPRGYPGQ-EDDnp | No Hydrolysis |
The following conditions for substrate hydrolysis were used: 50 mM Tris-HCl, pH 8.0. All the reactions were at 37°C. The arrow (↓) indicates the cleaved peptide bonds determined by fragment identification by LC/MS. *Both cleavage sites were equally hydrolyzed at the PAR-1 peptide.