Figure 2. Tks adaptor proteins are required for melanoma metastasis in vivo.
C57Bl/6 mice were injected intravenously with 1 × 105 B16F10 cells with Scr, Tks4 or Tks5 knockdown. After 28 days, the number of metastases per lung and size of each lung metastasis were evaluated. A. Representative macroscopic views of lung (top panels) and H&E-stained lung sections (middle and bottom panels). Arrowheads indicate metastatic melanoma in the lung, and high-magnification images (bottom panels) are shown from the indicated area in the middle panels. B. The number of lung metastases per lung and the size of each lung metastasis were determined by serial sections of each lung with H&E-staining. *p < 0.005, **p < 0.001, ***p < 10−4. Data are presented as means ± SEM; for (B, left) n = 10 for each group; for (B, right) n = Scr: 122, Tks4: 36, Tks5: 57. Scale bar: 1 mm (middle panel), 200μm (lower panel).