Table 1. Antibodies used.
| Antibody | Manufacturer | Clone | Catalog no | Application | Concentration |
|---|---|---|---|---|---|
| Ms α Phospho EGFR Y1068 | R&D Systems | 338324 | MAB3570 | WB | 1:500 |
| Rb α Phospho EGFR Y1173 | Cell Signaling | 53A5 | 4407 | WB | 1:500 |
| Rb α Phospho Stat3 Y705 | Cell Signaling | D3A7 | 9145 | WB | 1:1000 |
| Ms α Stat3 | Cell Signaling | 124H6 | 9139 | WB | 1:1000 |
| GAPDH | Millipore | 6C5 | MAB374 | WB | 1:10000 |
| Dk α Rb HRP | GE Healthcare | NA9310V | WB | 1:5000 | |
| Dk α Ms HRP | GE Healthcare | NA934V | WB | 1:5000 | |
| Ms α hEGFR | Dako | H11 | M3563 | IF, IHC | 1:200 |
| Isolectin IB4-568 | ThermoFisher Scientific | I21412 | IF and live | 1:200 | |
| Rb α EGFR | Santa Cruz Biotechnology | Sc-03 | WB | 1:200 | |
| Ms α Nestin | EMD Millipore | rat401 | MAB353 | IF | 1:200 |
| Rb α GFAP | Dako | Z0334 | IF | 1:400 | |
| Ms α Tuj1 | Covance | PRB435P | IF | 1:400 | |
| Rb α Olig2 | EMD Millipore | AB9610 | IF | 1:500 | |
| Ms α O4 | EMD Millipore | MAB345 | IF | 1:100 | |
| Gt α Rb Alexa 488 | ThermoFisher Scientific | A-11008 | IF | 1:500 | |
| Gt α Ms Alexa 568 | ThermoFisher Scientific | A-11004 | IF | 1:500 |
Abbreviations: Ms, mouse; Rb, rabbit; Dk, donkey; Gt, goat; WB, Western blotting; IF, immunofluorescence; IHC, immunohistochemistry