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. 2016 Oct 15;7(48):79372–79387. doi: 10.18632/oncotarget.12676

Figure 3. N-Myc regulates the expression of differentiation-inducing miRNAs.

Figure 3

A. IPA analysis identifies the potential mechanisms by whichN-Myc regulates the expression of the ten differentiation-inducing miRNAs. B-C. MYCN mRNA levels and N-Myc protein expression as a function of MYCN depletion. Cells were transfected with MYCN siRNA (si-MYCN) or control oligos for three days. (B) RNA was isolated for measuring MYCN mRNA levels as above, and (C) cell lysates were harvested for measuring N-Myc protein levels by Western blots as above. *, p<0.05 compared to control. D-F. Effect of MYCN knockdown on the expression of miRNAs. Cells were transfected with 25 nM of MYCN siRNA or control oligos. After 72 hours, RNA was collected, and miRNA levels were measured by qRT-PCR as above.