Table 1. MTT assay of ANG-TAT-PTX in tumor cells, compared with that of ANG-PTX.
| U87 glioblastoma cells | |||||
|---|---|---|---|---|---|
| Time | Free PTX | ANG-PTX | ANG | ANG-TAT-PTX | ANG-TAT |
| 24h | 20.53±2.21 | 33.21±3.32 | 8.44±0.86 | 31.65±3.28 | 10.19±1.19 |
| 48h | 42.36±2.35 | 50.24±4.75 | 14.65±1.29 | 73.53±6.45 | 16.53±1.73 |
Condition: The cells (1 × 105cells/100 μl/well) were seeded in 96-well plates at 37°C and 5% CO2. The aqueous solutions of samples were added in the culture medium following MTT assay protocol.
Legend: In MTT assay, the ANG-TAT-PTX exhibited an improved anti-tumor activity compared to that of ANG-PTX, suggesting the presence of TAT fragment increased the cellular uptake of PTX.