3-Amino-1,6-diethyl-2,5,7-trimethyl-4,4-diphenyl-4-bora-3a,4a-diaza-s-indacene displays solvent-dependent behaviour in both NMR and fluorescence spectroscopy.
Keywords: crystal structure, BODIPY, excitation and emission, fluorescence, NMR spectroscopy, solvent dependence
Abstract
In the title compound (3-amino-4,4-diphenyl-BODIPY), C28H32BN3, the central six-membered ring has a flattened sofa conformation, with one of the N atoms deviating by 0.142 (4) Å from the mean plane of the other five atoms, which have an r.m.s. deviation of 0.015 Å. The dihedral angle between the two essentially planar outer five-membered rings is 8.0 (2)°. In the crystal, molecules are linked via weak N—H⋯π interactions, forming chains along [010]. The compound displays solvent-dependent behaviours in both NMR and fluorescence spectroscopy. In the 1H NMR spectra, the aliphatic resonance signals virtually coalesce in solvents such as chloroform, dichloromethane and dibromoethane; however, they are fully resolved in solvents such as dimethyl sulfoxide (DMSO), methanol and toluene. The excitation and fluorescence intensities in chloroform decreased significantly over time, while in DMSO the decrease is not so profound. In toluene, the excitation and fluorescent intensities are not time-dependent. This behaviour is presumably attributed to the assembly of 3-amino-4,4-diphenyl-BODIPY in solution that leads to the formation of noncovalent structures, while in polar or aromatic solvents, the formation of these assemblies is disrupted, leading to resolution of signals in the NMR spectra.
Chemical context
4,4-Difluoro-3a,4a-diaza-4-bora-s-indacene (BODIPY, see Scheme 1), as an attractive fluorophore, has found many applications in material sciences, as sensors and in labelling biomolecules such as proteins, lipids and nucleic acids (Ulrich et al., 2008 ▸; Loudet & Burgess, 2007 ▸; Ziessel et al., 2007 ▸; Tram et al., 2011 ▸; Lu et al., 2014 ▸; Bessette & Hanan, 2014 ▸). In our efforts to develop new BODIPY labelling chemistry, BODIPY analogues bearing an amino group, such as 3-amino-4,4-difluoro- and 3-amino-4,4-diphenyl-BODIPY, are being sought. While 3-amino-4,4-difluoro-BODIPY has been synthesized previously (Liras et al., 2007 ▸), a unique solvent-dependent behaviour of 3-amino-4,4-diphenyl-BODIPY, but not 3-amino-4,4-difluoro-BODIPY, was observed by NMR. In this regard, the resonance signals of the aliphatic protons are fully resolved in solvents such as DMSO-d 6, but coalesced in solvents such as CDCl3. We herein report the solvent-dependent behaviour of 3-amino-4,4-diphenyl-BODIPY analogues as observed in the 1H NMR and in excitation and emission spectroscopy. The crystal structure suggests that the title compound could form noncolvalent assemblies in solvents such as CDCl3, leading to its solvent-dependent behaviours in NMR and fluorescence spectroscopy.
Synthesis of BODIPY 2b
The presence of an amino group in BODIPY allows for functional-group transformation and potential applications in labelling biomolecules. Towards the synthesis of amino BODIPY, an intriguing chemistry was recently described (Liras et al., 2007 ▸). In this chemistry, a one-pot reaction of a substituted pyrrole in the presence of sodium nitrite, acetic acid and acetic anhydride, followed by treatment with boron trifluoride dietherate, led to the formation of a mixture of amino 2a and acetimido BODIPY 3a (see Scheme 2, R = F). Following this approach, 3-amino-1,6-diethyl-2,5,7-trimethyl-4,4-diphenyl-3a,4a-diaza-4-bora-s-indacene (BODIPY 2b, see Scheme 2 and Fig. 1 ▸) was synthesized in very low yield (typically <5%), where boron trifluoride diethyl etherate was replaced with diphenylboron bromide (Scheme 2, R = Ph).
Figure 1.
The molecular structure of the title compound, with displacement ellipsoids drawn at the 30% probabilty level. H atoms are not shown.
Solvent-dependent behaviour of BODIPY 2b observed by NMR spectroscopy
The characterization of 2b by 1H NMR spectroscopy yielded intriguing results. While the proton signals in 1H NMR spectra are fully resolved in DMSO-d 6 (as in Fig. 2 ▸ f), the aliphatic protons are completely coalesced in CDCl3. It is also observed that gradual addition of CDCl3 to a solution of 2b in DMSO-d 6 led to a loss of resolution of the aliphatic protons (Figs. 2 ▸ b–e).
Figure 2.
1H NMR spectra of BODIPY 2 b in DMSO-d 6 or mixtures of CDCl3 and DMSO-d 6 in varying ratios: (a) DMSO-d 6/CDCl3 (1:2 v/v); (b) DMSO-d 6/CDCl3 (1:1 v/v); (c) DMSO-d 6/CDCl3 (5:2 v/v); (d) DMSO-d 6/CDCl3 (5:1 v/v); (e) DMSO-d 6/CDCl3 (10:1 v/v); (f) neat DMSO-d 6.
In deuterated dichloromethane and 1,2-dibromoethane, the 1H NMR spectra are similarly coalesced (data not shown). On the other hand, spectra are resolved in deuterated methanol and toluene (data not shown), despite the poor solubility of 2b in methanol. These observations prompted us to further investigate the absorption and fluorescent emission behaviour of BODIPY 2b in solution.
Solvent-dependent behavior of BODIPY 2b observed by fluorescence spectroscopy
Fig. 3 ▸(a) suggests that the fluorescence spectra of 2b in chloroform, and to some extend in DMSO as well, shows time-dependent fluorescent intensities. In contrast, most solvatochromic BODIPY fluorophores that have been reported in the literature often show different maximal emission wavelengths (Baruah et al., 2006 ▸; Clemens et al., 2008 ▸; Filarowski et al., 2010 ▸, 2015 ▸; de Rezende et al., 2014 ▸), however, those solvatochromic BODIPY dyes do not display a time-dependent change in fluorescent intensity.
Figure 3.
Excitation and emission profile of 3-amino-4,4-diphenyl-BODIPY 2 b in (a) chloroform, DMSO and toluene; (b) chloroform over 45 min; (c) DMSO over 45 min; (d) toluene over 60 min.
On the other hand, time-dependent spectroscopic changes, in emission intensity, shift of maximal emission wavelength, or absorbance, have been observed for compounds that undergo self-assembly in solution (Gassensmith et al., 2007 ▸; Miyatake et al., 2005 ▸). Taken together, these observations suggest that BODIPY 2b shows a tendency to form assembled structures in chloroform, not as significantly in DMSO, and particularly not in toluene.
It can be seen from the crystal structure of BODIPY 2b that the molecules are linked along the BODIPY plane by interactions between one of the amino H atoms and the BODIPY π ring (N—H⋯π ring; Table 1 ▸ and Fig. 4 ▸).
Table 1. Hydrogen-bond geometry (Å, °).
Cg1 and Cg2 are the centroids of the C17–C22 and N2/C6–C9 rings, respectively.
| D—H⋯A | D—H | H⋯A | D⋯A | D—H⋯A |
|---|---|---|---|---|
| N3—H1N⋯Cg1 | 0.87 (4) | 3.07 (3) | 3.772 (2) | 139 (2) |
| N3—H2N⋯Cg2i | 0.87 (4) | 2.44 (3) | 3.223 (2) | 150 (2) |
Symmetry code: (i) 
.
Figure 4.
Part of the crystal structure of 2b, with weak C—H⋯π interactions shown as dashed lines.
It is conceivable that in solutions such as in dichloromethane, chloroform and dibromoethane, compound 2b could maintain similar intermolecular assemblies. As a consequence of the reduced mobility of the BODIPY molecules in these assembled structures, the alkyl signals are broadened to the extent that they become invisible in the NMR spectra (Celis et al., 2013 ▸; Brand et al., 2008 ▸; Chen et al., 2015 ▸). Motion of the phenyl rings, however, is not affected in the assembly, and thus the phenyl aromatic protons are visible in these solvents. In polar solvents such as DMSO and methanol, it is possible that solvation of the BODIPY NH2 group abolishes the ability for such assemblies to occur. On the other hand, in toluene, strong interactions of the aromatic benzene ring with the BODIPY co-plane could also diminish the assemblies. The emission profiles of BODIPY 2b in DMSO, chloroform and toluene also corroborate this model.
Structural commentary
The molecular structure of 2b shown in Fig. 1 ▸ displays a typical BODIPY structure (Tram et al., 2009 ▸). The central six-membered ring has a flattened sofa conformation with atom N1 deviating by 0.142 (4) Å from the mean plane of the other five atoms (N2/C4/C5/C6/N1), which has an r.m.s. deviation of 0.015 Å. The dihedral angle between the two essentailly planar outer five-membered rings (N1/C1–C4 and N2/C6–C9) is 8.0 (2)°. The two B—N bond lengths are the same within experimental error [1.594 (4) and 1.579 (4) Å], confirming the delocalized nature of the BODIPY core. The two phenyl rings form dihedral angles of 78.8 (1) (C17–C22) and 80.8 (1)° (C23–C28) with the approximate plane of the 12 atoms of the BODIPY core (B1/N1/N2/C1–C9), which has an r.m.s. deviation of 0.067 Å. The dihedral angle between the two phenyl rings is 48.6 (2)°. Methyl atoms C12 and C15, belonging to the ethyl substituents, deviate by −1.326 (4) and 1.348 (3) Å, respectively, from the mean plane of the 12 atoms of the BODIPY core. There is a weak intramolecular N3—H1N⋯π interaction involving the amino group and the C17–C22 phenyl ring (Table 1 ▸).
Supramolecular features
In the crystal, molecules are linked via weak N—H⋯π interactions (Table 1 ▸), forming chains along [010] (Fig. 4 ▸).
Spectroscopy and experimental
Bruker Avance 300 and 600 Digital NMR spectrometers with a 14.1 and 7.05 Tesla Ultrashield magnet, respectively, were used to obtain 1H and 11B NMR spectra. 1H NMR spectra were measured at 300 or 600 MHz, and 11B at 96 MHz. Chemical shifts and coupling constants (J values) are given in ppm (δ) and Hz, respectively. Deuterated solvents were purchased from C/D/N Isotopes Inc. Fluorescence spectroscopy was recorded using a QuantaMaster model QM-2001-4 cuvette-based L-format scanning spectrofluorometer from Photon Technology International (PTI), interfaced with FeliX32 software. UV–Vis spectra were obtained using a Thermospectronic/Unicam UV/Vis spectrometer configured to the Vision32 software.
Anhydrous dichloromethane, triethylamine and toluene were generated by first heating under reflux in the presence of phosphorus pentoxide, calcium hydride and sodium metal, respectively, followed by distillation under an atmosphere of nitrogen. All other chemicals and reagents were purchased from Sigma–Aldrich or TCI without further purification prior to use.
Synthesis and crystallization
For the preparation of 2b, a solution of sodium nitrite (80 mg, 1.2 mmol) in water (1.0 ml) was added dropwise to another solution of 3-ethyl-2,4-dimethylpyrrole (0.25 ml, 1.85 mmol) in acetic acid (7.5 ml) and acetic anhydride (7.5 ml). The mixture was then heated at 373 K for 4 h. The solvents were removed under reduced pressure. The resulting products were diluted with dichloromethane (20 ml) and washed with a saturated aqueous sodium bicarbonate solution (2 × 15 ml). The organic phase was dried (MgSO4) and evaporated to dryness under reduced pressure. The residue was co-evaporated with dry toluene (10 ml) and then redissolved in dry dichloromethane (10 ml), followed by addition of dry triethylamine (1.0 ml, 7.1 mmol). After stirring for 30 min, boron–diphenylbromide (Noth & Vahrenkamp, 1968 ▸) (1.5 ml, 8.2 mmol) was added. Stirring was continued for 20 h and the products were washed with water (3 × 30 ml), dried (MgSO4) and evaporated under reduced pressure. The residue was purified by column chromatography on silica gel. The appropriate fractions, eluted with dichloromethane–hexane (1:9 v/v), were pooled and concentrated under reduced pressure to give the title compound as an orange solid (yield 18 mg, 4%). Single crystals were obtained by slow evaporation of the corresponding solution in hexane. δH[DMSO-d 6]: 7.19–7.64 (br, 10H), 6.89 (s, 1H), 5.94 (br, 2H), 2.55 (q, 2H, J = 7.5), 2.27 (q, 2H, J = 7.5 Hz), 2.13 (s, 3H), 1.83 (s, 3H), 1.50 (s, 3H), 1.09 (t, 2H, J = 7.5 Hz), 0.93 (t, 2H, J = 7.5 Hz). δB[DMSO-d 6]: 0.66 (s).
Refinement
Crystal data, data collection and structure refinement details are summarized in Table 2 ▸. H atoms bonded to C atoms were included in calculated positions, with C—H = 0.95–0.99 Å, and were allowed to refine in a riding-motion approximation, with U iso(H) = 1.2U eq(C) or 1.5U eq(Cmethyl). The amino H atoms were refined independently with isotropic displacement parameters.
Table 2. Experimental details.
| Crystal data | |
| Chemical formula | C28H32BN3 |
| M r | 421.37 |
| Crystal system, space group | Monoclinic, P21 |
| Temperature (K) | 147 |
| a, b, c (Å) | 9.4938 (7), 11.5325 (8), 11.3739 (9) |
| β (°) | 109.557 (2) |
| V (Å3) | 1173.45 (15) |
| Z | 2 |
| Radiation type | Mo Kα |
| μ (mm−1) | 0.07 |
| Crystal size (mm) | 0.35 × 0.27 × 0.07 |
| Data collection | |
| Diffractometer | Bruker Kappa APEX DUO CCD |
| Absorption correction | Multi-scan (SADABS; Bruker, 2014 ▸) |
| T min, T max | 0.701, 0.746 |
| No. of measured, independent and observed [I > 2σ(I)] reflections | 10457, 5032, 4054 |
| R int | 0.040 |
| (sin θ/λ)max (Å−1) | 0.650 |
| Refinement | |
| R[F 2 > 2σ(F 2)], wR(F 2), S | 0.046, 0.104, 1.03 |
| No. of reflections | 5032 |
| No. of parameters | 302 |
| No. of restraints | 1 |
| H-atom treatment | H atoms treated by a mixture of independent and constrained refinement |
| Δρmax, Δρmin (e Å−3) | 0.19, −0.19 |
| Absolute structure | Flack x determined using 1500 quotients [(I +)−(I −)]/[(I +)+(I −)] (Parsons et al., 2013 ▸) |
| Absolute structure parameter | −1.3 (10) |
Supplementary Material
Crystal structure: contains datablock(s) I. DOI: 10.1107/S2056989017002213/sj5520sup1.cif
Structure factors: contains datablock(s) I. DOI: 10.1107/S2056989017002213/sj5520Isup2.hkl
CCDC reference: 1531986
Additional supporting information: crystallographic information; 3D view; checkCIF report
Acknowledgments
This work was supported by the Natural Sciences and Engineering Research Council of Canada.
supplementary crystallographic information
Crystal data
| C28H32BN3 | F(000) = 452 |
| Mr = 421.37 | Dx = 1.193 Mg m−3 |
| Monoclinic, P21 | Mo Kα radiation, λ = 0.71073 Å |
| a = 9.4938 (7) Å | Cell parameters from 4278 reflections |
| b = 11.5325 (8) Å | θ = 2.4–27.5° |
| c = 11.3739 (9) Å | µ = 0.07 mm−1 |
| β = 109.557 (2)° | T = 147 K |
| V = 1173.45 (15) Å3 | Plate, red |
| Z = 2 | 0.35 × 0.27 × 0.07 mm |
Data collection
| Bruker Kappa APEX DUO CCD diffractometer | 4054 reflections with I > 2σ(I) |
| Radiation source: sealed tube with Bruker Triumph monochromator | Rint = 0.040 |
| φ and ω scans | θmax = 27.5°, θmin = 1.9° |
| Absorption correction: multi-scan (SADABS; Bruker, 2014) | h = −12→12 |
| Tmin = 0.701, Tmax = 0.746 | k = −14→11 |
| 10457 measured reflections | l = −14→14 |
| 5032 independent reflections |
Refinement
| Refinement on F2 | Hydrogen site location: mixed |
| Least-squares matrix: full | H atoms treated by a mixture of independent and constrained refinement |
| R[F2 > 2σ(F2)] = 0.046 | w = 1/[σ2(Fo2) + (0.0464P)2 + 0.0459P] where P = (Fo2 + 2Fc2)/3 |
| wR(F2) = 0.104 | (Δ/σ)max < 0.001 |
| S = 1.03 | Δρmax = 0.19 e Å−3 |
| 5032 reflections | Δρmin = −0.19 e Å−3 |
| 302 parameters | Absolute structure: Flack x determined using 1500 quotients [(I+)-(I-)]/[(I+)+(I-)] (Parsons et al, 2013) |
| 1 restraint | Absolute structure parameter: −1.3 (10) |
Special details
| Geometry. All e.s.d.'s (except the e.s.d. in the dihedral angle between two l.s. planes) are estimated using the full covariance matrix. The cell e.s.d.'s are taken into account individually in the estimation of e.s.d.'s in distances, angles and torsion angles; correlations between e.s.d.'s in cell parameters are only used when they are defined by crystal symmetry. An approximate (isotropic) treatment of cell e.s.d.'s is used for estimating e.s.d.'s involving l.s. planes. |
Fractional atomic coordinates and isotropic or equivalent isotropic displacement parameters (Å2)
| x | y | z | Uiso*/Ueq | ||
| N1 | 0.6209 (2) | 0.4011 (2) | 0.47877 (19) | 0.0171 (5) | |
| N2 | 0.7753 (2) | 0.51637 (19) | 0.66391 (19) | 0.0174 (5) | |
| N3 | 0.4280 (3) | 0.2624 (2) | 0.4350 (3) | 0.0291 (6) | |
| C1 | 0.5218 (3) | 0.3302 (3) | 0.4010 (3) | 0.0204 (6) | |
| C2 | 0.5273 (3) | 0.3383 (3) | 0.2759 (3) | 0.0223 (6) | |
| C3 | 0.6299 (3) | 0.4212 (3) | 0.2787 (2) | 0.0191 (6) | |
| C4 | 0.6900 (3) | 0.4631 (2) | 0.4057 (2) | 0.0174 (6) | |
| C5 | 0.7876 (3) | 0.5506 (2) | 0.4559 (2) | 0.0187 (6) | |
| H5A | 0.8298 | 0.5927 | 0.4041 | 0.022* | |
| C6 | 0.8277 (3) | 0.5803 (2) | 0.5833 (2) | 0.0172 (6) | |
| C7 | 0.9196 (3) | 0.6704 (2) | 0.6497 (3) | 0.0192 (6) | |
| C8 | 0.9259 (3) | 0.6587 (2) | 0.7743 (3) | 0.0208 (6) | |
| C9 | 0.8385 (3) | 0.5641 (3) | 0.7804 (2) | 0.0202 (6) | |
| C10 | 0.4334 (4) | 0.2669 (3) | 0.1690 (3) | 0.0348 (8) | |
| H10A | 0.4545 | 0.2885 | 0.0933 | 0.052* | |
| H10B | 0.3275 | 0.2805 | 0.1566 | 0.052* | |
| H10C | 0.4566 | 0.1846 | 0.1871 | 0.052* | |
| C11 | 0.6694 (3) | 0.4692 (3) | 0.1714 (3) | 0.0243 (7) | |
| H11A | 0.6559 | 0.4081 | 0.1075 | 0.029* | |
| H11B | 0.7760 | 0.4924 | 0.2009 | 0.029* | |
| C12 | 0.5734 (4) | 0.5736 (3) | 0.1125 (3) | 0.0434 (9) | |
| H12A | 0.6030 | 0.6028 | 0.0433 | 0.065* | |
| H12B | 0.5873 | 0.6348 | 0.1753 | 0.065* | |
| H12C | 0.4680 | 0.5506 | 0.0811 | 0.065* | |
| C13 | 0.9925 (3) | 0.7628 (3) | 0.5969 (3) | 0.0279 (7) | |
| H13A | 1.0991 | 0.7672 | 0.6462 | 0.042* | |
| H13B | 0.9451 | 0.8377 | 0.5998 | 0.042* | |
| H13C | 0.9810 | 0.7439 | 0.5102 | 0.042* | |
| C14 | 1.0172 (3) | 0.7322 (3) | 0.8821 (3) | 0.0260 (7) | |
| H14A | 0.9688 | 0.7331 | 0.9469 | 0.031* | |
| H14B | 1.0193 | 0.8129 | 0.8530 | 0.031* | |
| C15 | 1.1776 (3) | 0.6883 (3) | 0.9400 (3) | 0.0365 (8) | |
| H15A | 1.2328 | 0.7394 | 1.0089 | 0.055* | |
| H15B | 1.2265 | 0.6880 | 0.8765 | 0.055* | |
| H15C | 1.1765 | 0.6094 | 0.9716 | 0.055* | |
| C16 | 0.8189 (3) | 0.5127 (3) | 0.8948 (3) | 0.0263 (7) | |
| H16A | 0.7133 | 0.5161 | 0.8876 | 0.039* | |
| H16B | 0.8787 | 0.5565 | 0.9684 | 0.039* | |
| H16C | 0.8521 | 0.4317 | 0.9032 | 0.039* | |
| C17 | 0.5151 (3) | 0.4325 (2) | 0.6603 (2) | 0.0182 (6) | |
| C18 | 0.4069 (3) | 0.5069 (3) | 0.5835 (3) | 0.0269 (7) | |
| H18A | 0.4245 | 0.5410 | 0.5136 | 0.032* | |
| C19 | 0.2748 (3) | 0.5331 (3) | 0.6051 (3) | 0.0355 (8) | |
| H19A | 0.2036 | 0.5835 | 0.5502 | 0.043* | |
| C20 | 0.2475 (3) | 0.4855 (3) | 0.7068 (3) | 0.0347 (8) | |
| H20A | 0.1579 | 0.5036 | 0.7228 | 0.042* | |
| C21 | 0.3512 (3) | 0.4115 (3) | 0.7851 (3) | 0.0337 (8) | |
| H21A | 0.3330 | 0.3783 | 0.8551 | 0.040* | |
| C22 | 0.4828 (3) | 0.3855 (3) | 0.7615 (3) | 0.0261 (7) | |
| H22A | 0.5528 | 0.3341 | 0.8161 | 0.031* | |
| C23 | 0.7586 (3) | 0.2907 (2) | 0.6849 (2) | 0.0177 (6) | |
| C24 | 0.9149 (3) | 0.2865 (3) | 0.7172 (3) | 0.0239 (6) | |
| H24A | 0.9667 | 0.3550 | 0.7096 | 0.029* | |
| C25 | 0.9962 (3) | 0.1863 (3) | 0.7597 (3) | 0.0301 (7) | |
| H25A | 1.1018 | 0.1870 | 0.7805 | 0.036* | |
| C26 | 0.9244 (4) | 0.0857 (3) | 0.7719 (3) | 0.0295 (7) | |
| H26A | 0.9801 | 0.0169 | 0.8011 | 0.035* | |
| C27 | 0.7705 (4) | 0.0853 (3) | 0.7414 (3) | 0.0276 (7) | |
| H27A | 0.7201 | 0.0160 | 0.7491 | 0.033* | |
| C28 | 0.6900 (3) | 0.1866 (3) | 0.6996 (3) | 0.0238 (6) | |
| H28A | 0.5846 | 0.1853 | 0.6803 | 0.029* | |
| B1 | 0.6672 (3) | 0.4083 (3) | 0.6269 (3) | 0.0181 (6) | |
| H2N | 0.359 (4) | 0.227 (3) | 0.376 (3) | 0.035 (10)* | |
| H1N | 0.417 (4) | 0.268 (3) | 0.508 (4) | 0.052 (12)* |
Atomic displacement parameters (Å2)
| U11 | U22 | U33 | U12 | U13 | U23 | |
| N1 | 0.0158 (11) | 0.0172 (13) | 0.0187 (11) | −0.0024 (9) | 0.0062 (9) | 0.0003 (9) |
| N2 | 0.0170 (11) | 0.0171 (13) | 0.0173 (11) | 0.0007 (9) | 0.0046 (9) | 0.0019 (9) |
| N3 | 0.0315 (15) | 0.0320 (17) | 0.0225 (14) | −0.0164 (12) | 0.0072 (12) | −0.0018 (12) |
| C1 | 0.0202 (14) | 0.0194 (17) | 0.0202 (14) | −0.0043 (11) | 0.0048 (11) | −0.0022 (11) |
| C2 | 0.0221 (14) | 0.0232 (18) | 0.0201 (14) | −0.0028 (12) | 0.0053 (11) | −0.0008 (12) |
| C3 | 0.0174 (13) | 0.0207 (17) | 0.0200 (13) | 0.0019 (11) | 0.0071 (11) | −0.0007 (11) |
| C4 | 0.0176 (13) | 0.0166 (17) | 0.0193 (14) | 0.0011 (11) | 0.0079 (11) | 0.0030 (10) |
| C5 | 0.0168 (13) | 0.0206 (16) | 0.0203 (13) | 0.0014 (11) | 0.0084 (11) | 0.0034 (11) |
| C6 | 0.0145 (13) | 0.0170 (16) | 0.0203 (13) | 0.0004 (10) | 0.0057 (10) | 0.0016 (11) |
| C7 | 0.0157 (13) | 0.0168 (17) | 0.0240 (14) | 0.0000 (11) | 0.0052 (11) | −0.0002 (11) |
| C8 | 0.0189 (13) | 0.0185 (18) | 0.0232 (15) | 0.0019 (11) | 0.0046 (11) | −0.0023 (12) |
| C9 | 0.0168 (13) | 0.0221 (17) | 0.0195 (14) | 0.0029 (11) | 0.0031 (11) | −0.0008 (11) |
| C10 | 0.042 (2) | 0.034 (2) | 0.0282 (16) | −0.0144 (15) | 0.0110 (14) | −0.0074 (15) |
| C11 | 0.0289 (15) | 0.0261 (18) | 0.0208 (15) | −0.0028 (13) | 0.0120 (12) | −0.0013 (11) |
| C12 | 0.055 (2) | 0.044 (2) | 0.0372 (19) | 0.0168 (17) | 0.0241 (17) | 0.0187 (17) |
| C13 | 0.0289 (16) | 0.0236 (18) | 0.0307 (16) | −0.0063 (13) | 0.0095 (13) | −0.0004 (13) |
| C14 | 0.0299 (17) | 0.0221 (18) | 0.0247 (15) | −0.0037 (12) | 0.0072 (13) | −0.0064 (12) |
| C15 | 0.0292 (17) | 0.040 (2) | 0.0307 (18) | −0.0052 (15) | −0.0023 (14) | −0.0083 (14) |
| C16 | 0.0303 (15) | 0.0278 (18) | 0.0209 (15) | −0.0040 (13) | 0.0086 (12) | −0.0006 (12) |
| C17 | 0.0170 (13) | 0.0158 (16) | 0.0217 (14) | −0.0020 (10) | 0.0062 (10) | −0.0024 (11) |
| C18 | 0.0237 (15) | 0.0267 (18) | 0.0306 (16) | 0.0032 (12) | 0.0094 (12) | 0.0051 (13) |
| C19 | 0.0257 (16) | 0.035 (2) | 0.0425 (19) | 0.0076 (14) | 0.0074 (14) | 0.0016 (15) |
| C20 | 0.0220 (15) | 0.035 (2) | 0.052 (2) | −0.0011 (13) | 0.0191 (15) | −0.0099 (15) |
| C21 | 0.0334 (18) | 0.040 (2) | 0.0364 (18) | −0.0022 (15) | 0.0229 (15) | 0.0010 (15) |
| C22 | 0.0236 (15) | 0.0269 (19) | 0.0281 (16) | 0.0017 (12) | 0.0090 (12) | 0.0027 (12) |
| C23 | 0.0215 (14) | 0.0186 (16) | 0.0144 (13) | −0.0013 (11) | 0.0079 (11) | −0.0025 (10) |
| C24 | 0.0230 (15) | 0.0221 (17) | 0.0279 (15) | −0.0006 (12) | 0.0103 (12) | −0.0002 (12) |
| C25 | 0.0235 (15) | 0.0296 (19) | 0.0366 (18) | 0.0065 (13) | 0.0094 (13) | −0.0004 (14) |
| C26 | 0.0336 (18) | 0.0218 (19) | 0.0304 (17) | 0.0104 (13) | 0.0071 (13) | 0.0037 (13) |
| C27 | 0.0345 (17) | 0.0174 (18) | 0.0305 (16) | 0.0002 (12) | 0.0103 (13) | 0.0019 (12) |
| C28 | 0.0206 (14) | 0.0246 (18) | 0.0249 (15) | −0.0028 (12) | 0.0057 (12) | 0.0019 (12) |
| B1 | 0.0190 (15) | 0.0184 (18) | 0.0170 (15) | −0.0018 (12) | 0.0060 (12) | 0.0017 (12) |
Geometric parameters (Å, º)
| N1—C1 | 1.334 (3) | C13—H13C | 0.9800 |
| N1—C4 | 1.413 (3) | C14—C15 | 1.529 (4) |
| N1—B1 | 1.594 (4) | C14—H14A | 0.9900 |
| N2—C9 | 1.374 (3) | C14—H14B | 0.9900 |
| N2—C6 | 1.392 (3) | C15—H15A | 0.9800 |
| N2—B1 | 1.579 (4) | C15—H15B | 0.9800 |
| N3—C1 | 1.335 (4) | C15—H15C | 0.9800 |
| N3—H2N | 0.87 (4) | C16—H16A | 0.9800 |
| N3—H1N | 0.87 (4) | C16—H16B | 0.9800 |
| C1—C2 | 1.445 (4) | C16—H16C | 0.9800 |
| C2—C3 | 1.358 (4) | C17—C22 | 1.396 (4) |
| C2—C10 | 1.492 (4) | C17—C18 | 1.398 (4) |
| C3—C4 | 1.446 (4) | C17—B1 | 1.635 (4) |
| C3—C11 | 1.498 (4) | C18—C19 | 1.389 (4) |
| C4—C5 | 1.360 (4) | C18—H18A | 0.9500 |
| C5—C6 | 1.411 (4) | C19—C20 | 1.382 (5) |
| C5—H5A | 0.9500 | C19—H19A | 0.9500 |
| C6—C7 | 1.404 (4) | C20—C21 | 1.379 (5) |
| C7—C8 | 1.405 (4) | C20—H20A | 0.9500 |
| C7—C13 | 1.501 (4) | C21—C22 | 1.396 (4) |
| C8—C9 | 1.386 (4) | C21—H21A | 0.9500 |
| C8—C14 | 1.505 (4) | C22—H22A | 0.9500 |
| C9—C16 | 1.496 (4) | C23—C28 | 1.403 (4) |
| C10—H10A | 0.9800 | C23—C24 | 1.405 (4) |
| C10—H10B | 0.9800 | C23—B1 | 1.626 (4) |
| C10—H10C | 0.9800 | C24—C25 | 1.383 (4) |
| C11—C12 | 1.523 (4) | C24—H24A | 0.9500 |
| C11—H11A | 0.9900 | C25—C26 | 1.377 (5) |
| C11—H11B | 0.9900 | C25—H25A | 0.9500 |
| C12—H12A | 0.9800 | C26—C27 | 1.384 (4) |
| C12—H12B | 0.9800 | C26—H26A | 0.9500 |
| C12—H12C | 0.9800 | C27—C28 | 1.391 (4) |
| C13—H13A | 0.9800 | C27—H27A | 0.9500 |
| C13—H13B | 0.9800 | C28—H28A | 0.9500 |
| C1—N1—C4 | 106.5 (2) | C8—C14—C15 | 112.5 (3) |
| C1—N1—B1 | 128.0 (2) | C8—C14—H14A | 109.1 |
| C4—N1—B1 | 125.2 (2) | C15—C14—H14A | 109.1 |
| C9—N2—C6 | 106.6 (2) | C8—C14—H14B | 109.1 |
| C9—N2—B1 | 127.5 (2) | C15—C14—H14B | 109.1 |
| C6—N2—B1 | 125.9 (2) | H14A—C14—H14B | 107.8 |
| C1—N3—H2N | 117 (2) | C14—C15—H15A | 109.5 |
| C1—N3—H1N | 122 (3) | C14—C15—H15B | 109.5 |
| H2N—N3—H1N | 118 (3) | H15A—C15—H15B | 109.5 |
| N1—C1—N3 | 123.9 (3) | C14—C15—H15C | 109.5 |
| N1—C1—C2 | 111.3 (2) | H15A—C15—H15C | 109.5 |
| N3—C1—C2 | 124.8 (3) | H15B—C15—H15C | 109.5 |
| C3—C2—C1 | 106.5 (2) | C9—C16—H16A | 109.5 |
| C3—C2—C10 | 129.6 (3) | C9—C16—H16B | 109.5 |
| C1—C2—C10 | 123.9 (3) | H16A—C16—H16B | 109.5 |
| C2—C3—C4 | 107.4 (2) | C9—C16—H16C | 109.5 |
| C2—C3—C11 | 127.9 (3) | H16A—C16—H16C | 109.5 |
| C4—C3—C11 | 124.6 (3) | H16B—C16—H16C | 109.5 |
| C5—C4—N1 | 120.9 (2) | C22—C17—C18 | 115.8 (3) |
| C5—C4—C3 | 130.7 (2) | C22—C17—B1 | 125.5 (2) |
| N1—C4—C3 | 108.3 (2) | C18—C17—B1 | 118.7 (2) |
| C4—C5—C6 | 121.6 (3) | C19—C18—C17 | 122.7 (3) |
| C4—C5—H5A | 119.2 | C19—C18—H18A | 118.6 |
| C6—C5—H5A | 119.2 | C17—C18—H18A | 118.6 |
| N2—C6—C7 | 109.5 (2) | C20—C19—C18 | 119.7 (3) |
| N2—C6—C5 | 120.9 (2) | C20—C19—H19A | 120.2 |
| C7—C6—C5 | 129.6 (3) | C18—C19—H19A | 120.2 |
| C6—C7—C8 | 106.2 (2) | C21—C20—C19 | 119.6 (3) |
| C6—C7—C13 | 126.7 (2) | C21—C20—H20A | 120.2 |
| C8—C7—C13 | 127.1 (2) | C19—C20—H20A | 120.2 |
| C9—C8—C7 | 107.6 (2) | C20—C21—C22 | 120.0 (3) |
| C9—C8—C14 | 126.4 (3) | C20—C21—H21A | 120.0 |
| C7—C8—C14 | 125.9 (3) | C22—C21—H21A | 120.0 |
| N2—C9—C8 | 110.0 (2) | C17—C22—C21 | 122.2 (3) |
| N2—C9—C16 | 122.6 (3) | C17—C22—H22A | 118.9 |
| C8—C9—C16 | 127.3 (2) | C21—C22—H22A | 118.9 |
| C2—C10—H10A | 109.5 | C28—C23—C24 | 115.5 (3) |
| C2—C10—H10B | 109.5 | C28—C23—B1 | 123.8 (2) |
| H10A—C10—H10B | 109.5 | C24—C23—B1 | 120.6 (2) |
| C2—C10—H10C | 109.5 | C25—C24—C23 | 122.5 (3) |
| H10A—C10—H10C | 109.5 | C25—C24—H24A | 118.7 |
| H10B—C10—H10C | 109.5 | C23—C24—H24A | 118.7 |
| C3—C11—C12 | 112.0 (3) | C26—C25—C24 | 120.1 (3) |
| C3—C11—H11A | 109.2 | C26—C25—H25A | 120.0 |
| C12—C11—H11A | 109.2 | C24—C25—H25A | 120.0 |
| C3—C11—H11B | 109.2 | C25—C26—C27 | 119.7 (3) |
| C12—C11—H11B | 109.2 | C25—C26—H26A | 120.2 |
| H11A—C11—H11B | 107.9 | C27—C26—H26A | 120.2 |
| C11—C12—H12A | 109.5 | C26—C27—C28 | 119.7 (3) |
| C11—C12—H12B | 109.5 | C26—C27—H27A | 120.1 |
| H12A—C12—H12B | 109.5 | C28—C27—H27A | 120.1 |
| C11—C12—H12C | 109.5 | C27—C28—C23 | 122.4 (3) |
| H12A—C12—H12C | 109.5 | C27—C28—H28A | 118.8 |
| H12B—C12—H12C | 109.5 | C23—C28—H28A | 118.8 |
| C7—C13—H13A | 109.5 | N2—B1—N1 | 104.3 (2) |
| C7—C13—H13B | 109.5 | N2—B1—C23 | 109.8 (2) |
| H13A—C13—H13B | 109.5 | N1—B1—C23 | 107.8 (2) |
| C7—C13—H13C | 109.5 | N2—B1—C17 | 110.4 (2) |
| H13A—C13—H13C | 109.5 | N1—B1—C17 | 107.6 (2) |
| H13B—C13—H13C | 109.5 | C23—B1—C17 | 116.1 (2) |
| C4—N1—C1—N3 | −175.8 (3) | C2—C3—C11—C12 | 89.8 (4) |
| B1—N1—C1—N3 | 9.8 (5) | C4—C3—C11—C12 | −85.5 (4) |
| C4—N1—C1—C2 | 3.0 (3) | C9—C8—C14—C15 | 91.3 (4) |
| B1—N1—C1—C2 | −171.4 (2) | C7—C8—C14—C15 | −85.5 (4) |
| N1—C1—C2—C3 | −2.6 (3) | C22—C17—C18—C19 | −0.2 (5) |
| N3—C1—C2—C3 | 176.2 (3) | B1—C17—C18—C19 | −179.6 (3) |
| N1—C1—C2—C10 | 177.9 (3) | C17—C18—C19—C20 | 0.6 (5) |
| N3—C1—C2—C10 | −3.3 (5) | C18—C19—C20—C21 | −0.6 (5) |
| C1—C2—C3—C4 | 1.0 (3) | C19—C20—C21—C22 | 0.2 (5) |
| C10—C2—C3—C4 | −179.5 (3) | C18—C17—C22—C21 | −0.3 (4) |
| C1—C2—C3—C11 | −175.0 (3) | B1—C17—C22—C21 | 179.0 (3) |
| C10—C2—C3—C11 | 4.5 (5) | C20—C21—C22—C17 | 0.3 (5) |
| C1—N1—C4—C5 | 173.9 (3) | C28—C23—C24—C25 | 0.6 (4) |
| B1—N1—C4—C5 | −11.4 (4) | B1—C23—C24—C25 | −176.0 (3) |
| C1—N1—C4—C3 | −2.3 (3) | C23—C24—C25—C26 | −0.1 (5) |
| B1—N1—C4—C3 | 172.3 (2) | C24—C25—C26—C27 | 0.0 (5) |
| C2—C3—C4—C5 | −175.0 (3) | C25—C26—C27—C28 | −0.4 (5) |
| C11—C3—C4—C5 | 1.1 (5) | C26—C27—C28—C23 | 1.0 (4) |
| C2—C3—C4—N1 | 0.8 (3) | C24—C23—C28—C27 | −1.0 (4) |
| C11—C3—C4—N1 | 176.9 (2) | B1—C23—C28—C27 | 175.4 (3) |
| N1—C4—C5—C6 | 2.2 (4) | C9—N2—B1—N1 | 175.5 (2) |
| C3—C4—C5—C6 | 177.5 (3) | C6—N2—B1—N1 | −6.1 (3) |
| C9—N2—C6—C7 | −2.0 (3) | C9—N2—B1—C23 | −69.2 (3) |
| B1—N2—C6—C7 | 179.3 (2) | C6—N2—B1—C23 | 109.2 (3) |
| C9—N2—C6—C5 | 177.7 (2) | C9—N2—B1—C17 | 60.1 (3) |
| B1—N2—C6—C5 | −1.0 (4) | C6—N2—B1—C17 | −121.5 (3) |
| C4—C5—C6—N2 | 3.9 (4) | C1—N1—B1—N2 | −174.3 (3) |
| C4—C5—C6—C7 | −176.5 (3) | C4—N1—B1—N2 | 12.3 (3) |
| N2—C6—C7—C8 | 1.5 (3) | C1—N1—B1—C23 | 69.0 (3) |
| C5—C6—C7—C8 | −178.1 (3) | C4—N1—B1—C23 | −104.5 (3) |
| N2—C6—C7—C13 | −176.8 (3) | C1—N1—B1—C17 | −57.0 (4) |
| C5—C6—C7—C13 | 3.5 (5) | C4—N1—B1—C17 | 129.6 (3) |
| C6—C7—C8—C9 | −0.4 (3) | C28—C23—B1—N2 | 161.5 (2) |
| C13—C7—C8—C9 | 177.9 (3) | C24—C23—B1—N2 | −22.2 (3) |
| C6—C7—C8—C14 | 176.9 (3) | C28—C23—B1—N1 | −85.4 (3) |
| C13—C7—C8—C14 | −4.8 (4) | C24—C23—B1—N1 | 90.9 (3) |
| C6—N2—C9—C8 | 1.8 (3) | C28—C23—B1—C17 | 35.3 (4) |
| B1—N2—C9—C8 | −179.6 (2) | C24—C23—B1—C17 | −148.4 (2) |
| C6—N2—C9—C16 | −174.8 (3) | C22—C17—B1—N2 | −104.1 (3) |
| B1—N2—C9—C16 | 3.8 (4) | C18—C17—B1—N2 | 75.2 (3) |
| C7—C8—C9—N2 | −0.9 (3) | C22—C17—B1—N1 | 142.6 (3) |
| C14—C8—C9—N2 | −178.1 (3) | C18—C17—B1—N1 | −38.1 (3) |
| C7—C8—C9—C16 | 175.5 (3) | C22—C17—B1—C23 | 21.8 (4) |
| C14—C8—C9—C16 | −1.7 (5) | C18—C17—B1—C23 | −158.9 (3) |
Hydrogen-bond geometry (Å, º)
Cg1 and Cg2 are the centroids of the C17-C22 and N2/C6-C9 rings, respectively.
| D—H···A | D—H | H···A | D···A | D—H···A |
| N3—H1N···Cg1 | 0.87 (4) | 3.07 (3) | 3.772 (2) | 139 (2) |
| N3—H2N···Cg2i | 0.87 (4) | 2.44 (3) | 3.223 (2) | 150 (2) |
Symmetry code: (i) −x+1, y−1/2, −z+1.
References
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Associated Data
This section collects any data citations, data availability statements, or supplementary materials included in this article.
Supplementary Materials
Crystal structure: contains datablock(s) I. DOI: 10.1107/S2056989017002213/sj5520sup1.cif
Structure factors: contains datablock(s) I. DOI: 10.1107/S2056989017002213/sj5520Isup2.hkl
CCDC reference: 1531986
Additional supporting information: crystallographic information; 3D view; checkCIF report