NEUROSCIENCE Correction for “Cocaine elicits autophagic cytotoxicity via a nitric oxide-GAPDH signaling cascade,” by Prasun Guha, Maged M. Harraz, and Solomon H. Snyder, which appeared in issue 5, February 2, 2016, of Proc Natl Acad Sci USA (113:1417–1422; first published January 19, 2016; 10.1073/pnas.1524860113).
The authors note that Fig. 1 appeared incorrectly. The corrected figure and its legend appear below.
Fig. 1.
Cocaine elicits autophagy in neuronal system. (A and B) Immunoblots and quantification assessing the protein levels of LC3-II in response to indicated concentrations of cocaine in cortical cultures (n = 3). (C) Immunoblot assessing the protein levels of LC3-II in response to indicated concentrations of cocaine ±100 nM bafilomycin-A1. (D) Immunoblots assessing the protein levels of p62 in response to indicated concentrations of cocaine in cortical cultures. (E) Quantification of p62 protein levels in response to cocaine in cortical cultures (n = 3). (F) Confocal imaging of LC3 puncta (red) in cortical neurons ± 100 μM cocaine. Neuronal nuclei were stained green with NeuN antibody. (G) Quantification of LC3-II puncta per neuron ± cocaine treatment. A total of 50 neurons were counted. (Scale bar: 10 μm.) Data are represented as mean ± SD; ***P < 0.001, one-way ANOVA. (H) Transmission electron microscopy (TEM) images of cortical neurons with different indicated concentrations of cocaine for 3 h. Arrowhead represents autophagic vesicle, N denotes nucleus. (Scale bar: 2 μm.) The red boxed area contains a representative autophagic vacuole at 25,000× magnification. Arrow indicates double membrane of autophagic vacuole at 60,000× magnification. (I) Quantification of autophagic vacuoles per neuron ± cocaine. A total of 30 neurons were counted. Data are represented as mean ± SD; ***P < 0.001, **P < 0.01, *P < 0.05, one-way ANOVA. (J) TEM images of adult brain lateral habenula injected with vehicle or 20 mg/kg cocaine (intraperitoneal) for 3 h. (Scale bar: 2 μm.) The red boxed area contains a representative autophagic vacuole at 60,000× magnification. (K) TEM images of mouse fetal brain cerebral cortex. Timed pregnant mice were injected with vehicle or 20mg/kg cocaine (intraperitoneal) for 3 h. (Scale bar: 2 μm.) The red boxed area contains representative autophagic vacuoles at 60,000× magnification.