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. 2016 Nov 2;7(49):80612–80632. doi: 10.18632/oncotarget.13016

Figure 5. Pygo2 ablation completely suppresses the upregulation of Prox1, but fails to decrease the overexpression of c-Myc during early intestinal tumorigenesis in Apc LOF mice.

Figure 5

(A, B) Immunostains for detecting tumor progression markers SOX9, PROX1 and C-MYC on intestinal tissue sections of compound Apc LOF (A) and Ctnnb1 GOF animals (B). (C) qRT-PCR analyses for the relative RNA expression of Sox9, Prox1 and C-myc. Each bar represents one animal. Each graph represents the mean of at least three independent experiments from RNA of mice with the indicated genotype. Significant difference for the mean expression level relative to the corresponding control group is marked * for P < 0.05, and ** for P < 0.01. (D) Immunohistochemical analyses of intestinal tissues from compound Pygo2 deficient Ctnnb1 GOF mice on day 34 and day 86 post induction. Sections were stained with indicated antibodies for detecting Wnt/ß-catenin targets and tumor progression markers. Note that microadenoma also do not express PROX1, but show overexpression of C-MYC in the adenomatous cells. Scale bars in the pictures represent 200 μm for all IHC. Inserts show the staining at higher magnification.