Table 3. TRPV1v3 expression in GBM samples and GSCs.
| A) | ||||||
|---|---|---|---|---|---|---|
| TRPV1 5'UTR variants | NB (n=3/3) | NHA (n=2/2) | Grade I (n=8/8) | Grade II (n=10/12) | Grade III (n=12/20) | Grade IV (n=38/44) |
| TRPV1v0 | 0.42 ± 0.4 | 0.22 ± 0.01 | 0.62 ± 0.22 | 0.34 ± 0.01 | 0.16 ± 0.01 | 0 |
| TRPV1v1 | 0.61 ± 0.09 | 0.72 ± 0.11 | 0.33 ± 0.12 | 0.26 ± 0.01 | 0.03 ± 0.01 | 0 |
| TRPV1v3 | 0.85 ± 0.12 | 0.77 ± 0.13 | 1.12 ± 0.20 | 0.81 ± 0.19 | 0.44 ± 0.1 | 0.43 ± 0.2 |
| TRPV1v4 | 0.64 ± 0.11 | 0.61 ± 0.08 | 0.16 ± 0.01 | 0.40 ± 0.02 | 0.22 ± 0.1 | 0 |
| B) | |||
|---|---|---|---|
| TRPV1v3 | (n= 6/44) | (n= 22/44) | (n=16/44) |
| Rel. Expr. | 0 | ≤ 0.50 | > 0.50 |
| % of samples | 13.6 | 50 | 36.4 |
| C) | |||
|---|---|---|---|
| TRPV1 5'UTR variants | HNPC (n=3/3) | GSC (n=2/4) | dGSC (n=4/4) |
| TRPV1v0 | 0.42 ± 0.02 | 0 | 0.23 ± 0.01 |
| TRPV1v1 | 0.33 ± 0.02 | 0 | 0.27 ± 0.01 |
| TRPV1v3 | 1.02 ± 0.13 | 0.25 ± 0.01 | 0.38 ± 0.02 |
| TRPV1v4 | 0.22 ± 0.01 | 0 | 0.15 ± 0.01 |
A) TRPV1 5'UTR variants relative expression (Rel. Exp) levels in Normal Brain (NB), Normal Human Astrocytes (NHA) and in glioma biopsies from grade I to IV. B) TRPV1v3 expression levels in GBM samples, subdivided in three categories: no TRPV1v3 expression (Rel. Expr. = 0), low TRPV1v3 (Rel. Expr. ≤ 0.50) and high TRPV1v3 (Rel. Expr. > 0.50). TRPV1v3 relative expression levels are indicated. C) TRPV1 5'UTR variants relative expression (Rel. Exp) levels in human normal progenitor cells (HNPC), glioma stem-like cells (GSC) and dGSC (differentiated glioma stem-like cells). n: indicates the number of samples analyzed. A, B) n: indicates the number of TRPV1 positive samples respect to the total samples analyzed. A-C) Data are represented as mean of TRPV1 relative expression ± S.D. of three-separated RT/PCR analysis. Relative values were normalized to GAPDH expression level.