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. 1990 Feb;87(4):1481–1485. doi: 10.1073/pnas.87.4.1481

Two versions of the gene encoding the 41-kilodalton subunit of the telomere binding protein of Oxytricha nova.

B J Hicke 1, D W Celander 1, G H MacDonald 1, C M Price 1, T R Cech 1
PMCID: PMC53499  PMID: 1689486

Abstract

Macronuclear chromosomes of the ciliated protozoan Oxytricha nova terminate with a single-stranded (T4G4)2 overhang. The (T4G4)2 telomeric overhang is tenaciously bound by a protein heterodimer. We have cloned and sequenced the gene encoding the 41-kDa subunit of this telomere binding protein. The predicted amino acid sequence comprises two distinct regions, a carboxyl-terminal two-thirds that is 23% lysine and bears similarity to histone H1 and an amino-terminal one-third containing a hydrophobic stretch of about 15 amino acids. Two macronuclear versions of the gene differ in nucleotide sequence at several positions, but the derived polypeptides differ only at a single position, Ser-110 or Ala-110. Both versions harbor a small intron. The existence of this intron demonstrates that, despite the elimination of 95% of the micronuclear genome from the developing macronucleus, at least some noncoding DNA is retained during macronuclear development of hypotrichous ciliates.

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Selected References

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