Table 2. Proposed structures and their molecular ions in MALDI spectra of N-glycans in the four cell lines.
No. | Calculated m/z | Relative intensity | Glycan structure | Calculated m/z (acetyl hydrazine modification) | Glycan structure (sialic) | |||
---|---|---|---|---|---|---|---|---|
GES-1 | SGC-7901 | HGC-27 | MGC-803 | |||||
1 | 1257.423 | 0.048±0.001 | 0.024±0.002 | 0.078±0.045 | 0.018±0.009 | - | - | |
2 | 1419.476 | 0.189±0.001 | 0.352±0.080 | 0.294±0.142 | 0.172±0.008 | - | - | |
3 | 1565.533 | - | 0.020 | 0.021 | 0.026±0.003 | - | - | |
4 | 1581.528 | 0.245±0.035 | 0.365±0.052 | 0.307±0.023 | 0.351±0.008 | - | - | |
5 | 1622.555 | - | - | 0.021 | 0.025±0.018 | - | - | |
6 | 1663.581 | - | 0.104±0.011 | 0.048 | 0.023±0.014 | - | - | |
7 | 1688.613 | 0.082±0.025 | ||||||
8 | 1743.581 | 1.000 | 1.000 | 1.000 | 1.000 | - | - | |
9 | 1809.639 | 0.059±0.022 | 0.624±0.046 | 0.199±0.044 | 0.043±0.0004 | - | - | |
10 | 1850.666 | - | - | 0.054±0.041 | - | - | - | |
11 | 1865.654 | - | 0.076±0.074 | 0.039±0.030 | - | - | - | |
12 | 1905.634 | 0.382±0.106 | 0.438±0.066 | 0.724±0.050 | 0.476±0.003 | - | - | |
13 | 1955.697 | 0.048±0.025 | 0.294±0.142 | 0.020 | - | - | - | |
14 | 1996.724 | - | - | 0.018 | - | - | - | |
15 | 2012.719 | - | 0.032±0.021 | 0.078±0.026 | 0.048 | - | - | |
16 | 2028.714 | 0.054±0.015 | 0.041±0.006 | - | - | - | ||
17 | 2067.687 | 0.043±0.007 | 0.045±0.007 | 0.083±0.005 | 0.044±0.003 | - | - | |
18 | 2101.755 | - | 0.053±0.038 | - | - | 2047.713 | ||
19 | 2174.772 | 0.036±0.020 | 0.199±0.072 | 0.216±0.089 | - | - | - | |
20 | 2215.798 | - | - | 0.021±0.007 | - | 2157.756 | ||
21 | 2231.793 | - | 0.020±0.016 | 0.026±0.018 | - | - | - | |
22 | 2247.813 | - | 0.015 | - | - | - | - | |
23 | 2320.829 | - | 0.051±0.038 | - | - | 2262.788 | ||
24 | 2377.851 | - | 0.010±0.001 | 0.038±0.003 | - | - | - | |
25 | - | - | 0.069 | 0.020 | - | 2319.809 | ||
26 | 2393.846 | 0.036±0.0002 | ||||||
27 | 2466.887 | - | 0.028 | - | - | - | - | |
28 | 2539.904 | - | 0.214±0.077 | 0.147±0.048 | - | - | - | |
29 | - | - | 0.017±0.014 | 0.015±0.012 | - | 2538.908 | ||
30 | 2612.945 | - | 0.009 | - | - | - | - | |
31 | 2685.962 | - | 0.036±0.027 | - | - | - | - | |
32 | 2758.978 | - | 0.006±0.001 | - | - | - | - | |
33 | 2832.02 | - | 0.013 | - | - | - | - | |
34 | 2905.036 | - | 0.011±0.003 | 0.020±0.006 | - | - | - | |
35 | - | - | 0.006 | - | - | 2993.052 |
N-glycans with SD were identified in two independent experiments, and N-glycans without SD were identified in only one experiment.