Figure 7. Knockout of Sema3E reduces tumor incidence and tumor growth in vivo.

Sema3E-knockout Panc28-S3EKO cells as well as the corresponding control Panc28-Ctrl cells (2 × 10⁶ cells) were orthotopically implanted into the pancreas of 6 week old nude mice (n=5 per group). Mice were sacrificed at 8 weeks post-implantation, and pancreas were explanted and analyzed. Mice injected with Panc28-S3EKO had reduced tumor incidence (only 2 out of 5 animals showed tumor growth) compared to the control (Panc28-Ctrl) (all 5 animals had tumor growth). Extracted tumors from the pancreas of these mice were measured, and tumor volume was calculated from measurements of length and width. Tumors from the Panc28-S3EKO group had significantly lower tumor volume than tumors from the Panc28-Ctrl group (Student's t-test, *p<0.05). All data are represented as mean ± S.E.M.