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. 2016 Dec 20;8(4):6155–6168. doi: 10.18632/oncotarget.14047

Figure 3. AKT isoform specific in vitro kinase assays.

Figure 3

AKT isoform specific kinase activity in parental MDA-MB-231 (WT) cells, its brain seeking (BR) subline cells and PTEN overexpressing MDA-MB-231 BR cells (BR/PTEN) was analyzed by immunoprecipitation of AKT isoforms followed by incubation with AKT substrate GSK3α/β fusion protein. Phosphorylation at S9/21 was detected by Western blot analysis. Relative AKT isoform activity was quantified from Western blot analysis of triplicates, normalized to the corresponding mouse IgG level for sample correction.