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. 2016 Dec 21;8(4):6319–6329. doi: 10.18632/oncotarget.14062

Figure 6. FK228 cooperates with ara-C or DNR in inducing apoptosis and abrogates S and/or G2/M cell cycle checkpoint activation induced by ara-C or DNR in THP-1 and OCI-AML3 AML cells.

Figure 6

(A) THP-1 cells were treated with FK228 and ara-C or DNR, alone or in combination, for 48 h and then subjected to Annexin V-FITC/PI staining and flow cytometry analyses. **indicates p < 0.01 and ***indicates p < 0.001. (B) Whole cell lysates were subjected to Western blotting and probed with the indicated antibodies. The fold changes for the densitometry measurements, normalized to β-actin and then compared to no drug treatment control, are indicated. (C) OCI-AML3 cells were treated with FK228 and ara-C or DNR, alone or in combination, for 48 h and then subjected to Annexin V-FITC/PI staining and flow cytometry analyses. ***indicates p < 0.001. (D) Whole cell lysates were subjected to Western blotting and probed with the indicated antibodies. The fold changes for the densitometry measurements, normalized to β-actin and then compared to no drug treatment control, are indicated. (E and F) THP-1 and OCI-AML3 cells were treated with FK228 and ara-C or DNR, alone or in combination for 48 h and then fixed with ethanol and stained with PI for cell cycle analysis.