Table 2. Relative quantification of miRNA normalized to U6snRNA in the frozen tissue and FFPE tissue of training cohort.

	CRC patients with recurrence, n=14		CRC patients without recurrence, n=66		Threshold	AUC
	median	range	median	range
Frozen Tissue
let-7a	0.16	0.051 – 0.40	0.095	0.011 – 0.55	0.181	0.680
let-7d	0.026	0.0081 – 0.069	0.015	0.0024 – 0.11	0.014	0.698
let-7e	0.26	0.072 – 0.78	0.19	0.034 – 1.24	0.451	0.672
miR-18b	1.7 × 10-5	0 – 4.5 × 10-4	4.6 × 10-6	0 – 1.3 × 10-4	2.7 × 10-5	0.669
miR-23c	0.0025	1.4 × 10-4 – 0.0081	9.9 × 10-4	8.4 × 10-5 – 0.0090	0.002	0.724
miR-26b	0.27	0.031 – 0.74	0.22	0.028 – 1.53	0.496	0.642
miR-128a	0.018	0.0037 – 0.076	0.012	0.0010 – 0.092	0.035	0.632
miR-146b	0.22	0.0096 – 1.21	0.22	0.026 – 0.76	0.116	0.535
miR-148b	0.014	0.0017 – 0.070	0.0075	0.0015 – 0.033	0.007	0.686
miR-151-5p	0.024	0.0027 – 0.17	0.017	0.0020 – 0.085	0.022	0.633
miR-181c	0.0069	8.3 × 10-4 – 0.022	0.0042	4.4 × 10-4 – 0.032	0.007	0.729
miR-221	0.14	0.045 – 1.08	0.11	0.018 – 0.71	0.444	0.483
miR-222	0.40	0.075 – 1.35	0.48	0.036 – 1.19	0.791	0.462
miR-361	0.013	0.0020 – 0.052	0.0075	8.1 × 10-4 – 0.063	0.009	0.682
miR-500	0.0032	5.3 × 10-4 – 0.019	0.0036	2.0 × 10-4 – 0.020	0.008	0.526
FFPE tissue
let-7a	0.23	0.096 – 0.55	0.20	0.051 – 0.68	0.245	0.542
let-7d	0.096	0.047 – 0.21	0.75	0.021 – 0.21	0.111	0.658
let-7e	0.31	0.16 – 0.59	0.25	0.083 – 0.63	0.303	0.618
miR-23c	0.012	0.0033 – 0.029	0.012	0.0059 – 0.019	0.023	0.557
miR-26b	0.21	0.10 – 0.43	0.16	0.049 – 0.43	0.174	0.668
miR-128a	0.0090	0.0037 – 0.020	0.0076	0.0024 – 0.026	0.008	0.641
miR-148b	0.0015	0.0059 – 0.019	0.0097	0.0029 – 0.040	0.010	0.675
miR-151-5p	0.024	0.0072 – 0.040	0.017	3.4 × 10-5 – 0.057	0.021	0.670
miR-181c	0.0091	0.0041 – 0.015	0.0053	0.0018 – 0.018	0.010	0.694
miR-361	0.0093	0.0027 – 0.016	0.0071	0.0015 – 0.021	0.009	0.617

CRC: colorectal cancer, AUC: area under the curve, The thresholds were established using ROC curve and Youden index.