Table 2. Relative quantification of miRNA normalized to U6snRNA in the frozen tissue and FFPE tissue of training cohort.
CRC patients with recurrence, n=14 | CRC patients without recurrence, n=66 | Threshold | AUC | |||
---|---|---|---|---|---|---|
median | range | median | range | |||
Frozen Tissue | ||||||
let-7a | 0.16 | 0.051 – 0.40 | 0.095 | 0.011 – 0.55 | 0.181 | 0.680 |
let-7d | 0.026 | 0.0081 – 0.069 | 0.015 | 0.0024 – 0.11 | 0.014 | 0.698 |
let-7e | 0.26 | 0.072 – 0.78 | 0.19 | 0.034 – 1.24 | 0.451 | 0.672 |
miR-18b | 1.7 × 10-5 | 0 – 4.5 × 10-4 | 4.6 × 10-6 | 0 – 1.3 × 10-4 | 2.7 × 10-5 | 0.669 |
miR-23c | 0.0025 | 1.4 × 10-4 – 0.0081 | 9.9 × 10-4 | 8.4 × 10-5 – 0.0090 | 0.002 | 0.724 |
miR-26b | 0.27 | 0.031 – 0.74 | 0.22 | 0.028 – 1.53 | 0.496 | 0.642 |
miR-128a | 0.018 | 0.0037 – 0.076 | 0.012 | 0.0010 – 0.092 | 0.035 | 0.632 |
miR-146b | 0.22 | 0.0096 – 1.21 | 0.22 | 0.026 – 0.76 | 0.116 | 0.535 |
miR-148b | 0.014 | 0.0017 – 0.070 | 0.0075 | 0.0015 – 0.033 | 0.007 | 0.686 |
miR-151-5p | 0.024 | 0.0027 – 0.17 | 0.017 | 0.0020 – 0.085 | 0.022 | 0.633 |
miR-181c | 0.0069 | 8.3 × 10-4 – 0.022 | 0.0042 | 4.4 × 10-4 – 0.032 | 0.007 | 0.729 |
miR-221 | 0.14 | 0.045 – 1.08 | 0.11 | 0.018 – 0.71 | 0.444 | 0.483 |
miR-222 | 0.40 | 0.075 – 1.35 | 0.48 | 0.036 – 1.19 | 0.791 | 0.462 |
miR-361 | 0.013 | 0.0020 – 0.052 | 0.0075 | 8.1 × 10-4 – 0.063 | 0.009 | 0.682 |
miR-500 | 0.0032 | 5.3 × 10-4 – 0.019 | 0.0036 | 2.0 × 10-4 – 0.020 | 0.008 | 0.526 |
FFPE tissue | ||||||
let-7a | 0.23 | 0.096 – 0.55 | 0.20 | 0.051 – 0.68 | 0.245 | 0.542 |
let-7d | 0.096 | 0.047 – 0.21 | 0.75 | 0.021 – 0.21 | 0.111 | 0.658 |
let-7e | 0.31 | 0.16 – 0.59 | 0.25 | 0.083 – 0.63 | 0.303 | 0.618 |
miR-23c | 0.012 | 0.0033 – 0.029 | 0.012 | 0.0059 – 0.019 | 0.023 | 0.557 |
miR-26b | 0.21 | 0.10 – 0.43 | 0.16 | 0.049 – 0.43 | 0.174 | 0.668 |
miR-128a | 0.0090 | 0.0037 – 0.020 | 0.0076 | 0.0024 – 0.026 | 0.008 | 0.641 |
miR-148b | 0.0015 | 0.0059 – 0.019 | 0.0097 | 0.0029 – 0.040 | 0.010 | 0.675 |
miR-151-5p | 0.024 | 0.0072 – 0.040 | 0.017 | 3.4 × 10-5 – 0.057 | 0.021 | 0.670 |
miR-181c | 0.0091 | 0.0041 – 0.015 | 0.0053 | 0.0018 – 0.018 | 0.010 | 0.694 |
miR-361 | 0.0093 | 0.0027 – 0.016 | 0.0071 | 0.0015 – 0.021 | 0.009 | 0.617 |
CRC: colorectal cancer, AUC: area under the curve, The thresholds were established using ROC curve and Youden index.