Table 4. miRNA expressions in the FFPE tissue and pathological characteristics compared with and without recurrence.
| Patients with recurrencen=14, (%) | Patients without recurrencen=66, (%) | Chi-square | Multivariate analysis for recurrence | 3 yearRFS rate (%) | log-rank | Multivariate analysis for RFS | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P value | Odds ratio | 95% C.I. | P value | P value | Hazard ratio | 95% C.I. | P value | ||||||
| let-7d | 0.004 | 0.249 | 0.004 | 0.174 | |||||||||
| High (n=17) | 7 | (50.0) | 10 | (15.2) | 70.6 | ||||||||
| Low (n=63) | 7 | (50.0) | 56 | (84.8) | 88.9 | ||||||||
| let-7e | 0.030 | 0.505 | 0.339 | 0.397 | |||||||||
| High (n=26) | 8 | (57.1) | 18 | (27.3) | 76.9 | ||||||||
| Low (n=54) | 6 | (42.9) | 48 | (72.7) | 88.9 | ||||||||
| miR-26b | 0.038 | 0.153 | 0.044 | 0.283 | |||||||||
| High (n=37) | 10 | (71.4) | 27 | (40.9) | 81.1 | ||||||||
| Low (n=43) | 4 | (28.6) | 39 | (59.1) | 90.7 | ||||||||
| miR-128a | 0.038 | 0.282 | 0.0438 | 0.285 | |||||||||
| High (n=37) | 10 | (71.4) | 27 | (40.9) | 81.1 | ||||||||
| Low (n=43) | 4 | (28.6) | 39 | (59.1) | 90.7 | ||||||||
| miR-148b | 0.006 | 0.173 | 0.007 | 0.079 | |||||||||
| High (n=42) | 12 | (85.7) | 30 | (45.5) | 78.6 | ||||||||
| Low (n=38) | 2 | (14.3) | 36 | (54.5) | 94.7 | ||||||||
| miR-151-5p | 0.012 | 0.341 | 0.117 | 0.252 | |||||||||
| High (n=28) | 9 | (64.3) | 19 | (28.8) | 77.2 | ||||||||
| Low (n=52) | 5 | (35.7) | 47 | (71.2) | 89.8 | ||||||||
| miR-181c | 0.001 | 7.46 | 1.97 – 28.57 | 0.003 | 0.001 | 4.74 | 1.66 – 13.51 | 0.004 | |||||
| High (n=15) | 7 | (50.0) | 8 | (12.1) | 66.7 | ||||||||
| Low (n=65) | 7 | (50.0) | 58 | (87.9) | 89.2 | ||||||||
| miR-361 | 0.056 | 0.694 | 0.076 | 0.894 | |||||||||
| High (n=28) | 8 | (57.1) | 20 | (30.3) | 79.8 | ||||||||
| Low (n=52) | 6 | (42.9) | 46 | (69.7) | 88.5 | ||||||||
| Tumor depth | 0.048 | 0.627 | 0.094 | 0.150 | |||||||||
| T4 (n=14) | 5 | (35.7) | 9 | (13.6) | 75.0 | ||||||||
| T3 (n=66) | 9 | (64.3) | 57 | (86.4) | 88.2 | ||||||||
| Histology | 0.022 | 0.070 | 0.002 | 0.066 | |||||||||
| Muc (n=3) | 2 | (14.3) | 1 | (1.5) | 33.3 | ||||||||
| W/D and M/D (n=77) | 12 | (85.7) | 65 | (98.5) | 88.3 | ||||||||
C.I.: confidence interval, W/D: well-differentiated adenocarcinoma, M/D: moderately differentiated adenocarcinoma, Muc: mucinous carcinoma, High: higher expression of miRNA than the threshold, Low: equal or lower expression of miRNA than the threshold. The differences were analyzed by chi-square test, log-rank test or Multivariate logistic regression analysis. P < 0.05 denotes statistically significant difference.