Table 2. Cases with additional copy number changes identified by MLPA compared to R-band karyotype.

Case	Age/gender	WHO 2008	Karyotype	Chromosomal abnormalities detected by MLPA	IPSS-R risk group	IPSS-R risk group by MLPA
164	58/M	RAEB-1	NR	+8	NR	Very high
90	39/M	RAEB-2	NR	+8	NR	Very high
72	59/F	RCMD	46,XX[1]	5q-	High	High
52	37/F	RAEB-1	46,XX[5]	−7	High	Very high
51	47/F	RCMD	46,XX[6]	−7	Intermediate	High
37	42/M	RAEB-1	46,XY[9]	7q-	High	High
184	63/M	RAEB-1	46,XY[14]	7q-	High	Very high
36	51/M	RCMD	46,XY[3]	7q-	Low	Intermediate
74	53/F	RAEB-2	46,XX[13]	+8	Very high	Very high
442	60/M	RAEB-2	46,XY[20]	11q+	High	Very high
212	37/F	RAEB-1	46,XX[18]	11q+	High	Very high
362	68/M	RCMD	46,XY[20]	12p-	Low	Low
227	77/M	RCMD	46,XY[20]	17p-	Low	Intermediate
44	32/M	RCMD	46,XY[20]	17p-	Low	Intermediate
18	50/M	RAEB-1	46,XY[20]	17q-	Intermediate	High
406	76/M	RCMD	46,XY[20]	−19	Low	Low
134	57/F	RCMD	46,XX[10]	20q-	Low	Low
218	42/M	RCMD	46,XY[20]	20q-	Low	Low
88	41/M	RAEB-2	46,XY[7]	+8/11q+	High	Very high
406	63/M	RAEB-2	46,XY[9]	+8/17p-	Very high	Very high
142	38/F	RCMD	46,XX[15]	5q-/17p−/−19	Low	Intermediate
141	62/M	RAEB-1	46,XY[14]	5q-/12p-/17p-/ -19/20q-	High	Very high
300	72/M	RAEB-1	45,XY,-Y[5]/46,XY[15]	17p-	Low	Intermediate
344	68/M	RAEB-2	47,XY,+9,i(17)(q10)[3]/ 46,XY,i(17)(q10)[17]/ 46,XY[2]	17p−/−19/20q-	High	Very high
40	39/M	RAEB-1	46,XY,del(5)(q23)[10]	5q-/17p-	Intermediate	Intermediate
150	73/M	RCMD	45,X,-Y[8]/46,XY[2]	5q-	Low	Intermediate
161	53/M	RAEB-2	47,XY,+8[20]	5q-/+8	Very high	High
277	40/F	RAEB-1	45,XX,-7[18]/46,XX[20]	−7/17p−/−19/20q-	Very high	Very high
225	28/M	MDS-U	47,XY,+8,14ps+[4]	+8/12p-/17p-	Intermediate	High
139	53/M	RCMD	48,XY,+8,+21[10]	−5q/+8	Intermediate	High
9	47/M	RCMD	47,XY,+8[2]/46,XY[18]	+8/11q+	Low	Low
94	47/M	RAEB-1	46,XY,del(20)(q12)[20]	11q+/20q-	High	High
115	52/M	RAEB-1	46,XY,+X,del(20)(q12)[2]/46,XY,del(20)(q12)[4]	7q-/20q-	High	Very high
190	71/F	RAEB-2	46,XX,5q-,12q-[5]	5q-/+8/+11q	Very high	Very high
320	66/F	RCMD	41-45,XX,inc[cp19]/ 46,XX[1]	5q-/7q-	High	High
32	44/M	RCMD	47,XY,-22,+mar1,+mar2[8]/46,XY[2]	+8	High	High
41	59/F	RAEB-2	45,X,-X,del(5)(q13q33),del(6)(q23),add(17)(p13),del(17)(q22)[9]/46,XX[1]	5q−/−7/12p-	Very high	Very high
54	60/M	RAEB-1	45,XY,add(1)(q44),del(5)(q13q33),-12,add(17)(p10)[2]/45,XY,del(5)(q13q33),-12,add(17)(p10)[10]/46,XY[8]	5q-/+8/11q+/ 12p-	Very high	Very high
113	62/M	RCMD	42-46,XY,-2,-4,-6,-11,-12,-13,-17,+3-4mar,inc[cp10]	5q−/−7/−17p	Very high	Very high
154	50/F	RAEB1	43-46,X,-X,-12,-15,-17,-20,+1-2mar,inc[cp4]/46,XX[6]	5q-/+8	Very high	Very high
233	54/M	RCMD	43,XY,-5,-9,-21[13]/ 46,XY[7]	−5/7q-/12p−/−19	High	High
236	35/M	RCMD	49,XY,+Y,+8,+9[4]/ 46,XY[10]	+8/−19/20q-	High	Very high
318	46/M	RCMD	44,XY,add(2)(p25),-3,-5[12]/46,XY[8]	−7	High	Very high