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. 2016 Nov 21;8(1):552–564. doi: 10.18632/oncotarget.13477

Figure 6. Analysis of BAZF and Notch targets following Bcl-6/BCoR silencing.

Figure 6

(A, B) BECs were transfected with control, Bcl-6 or BAZF siRNA. Cell extracts were prepared 8 to 48 h after transfection. mRNA expression levels of Bcl-6 (A) and BAZF (B) were determined by real-time PCR in relation to cells transfected with control siRNA. Mean values and standard deviations from 2 independent experiments are given. (C) Sprouting assay of BECs transfected with Bcl-6 or BCoR siRNA with or without BAZF siRNA. A minimum of 19 spheroids were evaluated per condition; the cumulative sprout length was compared (T-Test; n.s.). (D) BECs transfected with Bcl-6 siRNA were harvested 24 h after electroporation. Transcript levels for components of the Notch pathway were determined via real-time PCR in relation to control siRNA treated cells (value set to 1). Mean values and standard deviations from 2-3 independent experiments are shown: Bcl-6, HES1, HEY1, HEY2, DLL4: N = 3; Notch1-4: N = 2. T-Test; *p < 0.05; **p < 0.01; ***p < 0.001.