Figure 5. Consequences of PSMC2 silencing on cell cycle progression and apoptosis in osteosarcoma cells.

a-b. Cell cycle was determined in SaoS-2 cells and MG-63 cells by flow cytometry five days after treatment with the indicated si-RNAs. The diagrams quantified cell fractions in the G₀/G₁, S and G₂/M fractions were shown. c-d. Apoptosis was determined by flow cytometry assays in two osteosarcoma cell lines with PSMC2 silence and control cells. The apoptotic rate was calculated as the percentage of Annexin FITC positive cells. Data were presented as mean ± SD from three independent experiments. **P < 0.01.