Figure 2. The C-terminus of Legless is required for nuclear Wg responses in imaginal discs.

(A, B) Third leg discs from third instar (A) wt or (B) lgs^2-8/lgs^2-8 larvae, fixed and stained with antibodies as indicated in panels (merges on the right), showing derepression of dpp.lacZ in the ventral compartment (arrow); space bar, 50 µm. (C, D) Corresponding wing discs, showing attenuated Sens expression along the prospective wing margin (see also insets); space bar, 100 µm. (E, F) Anterior margin segments of escaper flies, focused on stout margin bristles; yellow arrows, chemosensory bristles; red arrows, missing stout bristles causing gaps that are occupied by ectopic chemosensory bristles. (G) RT-qPCR assays of wing discs dissected from climbing wt and lgs^2-8/lgs^2-8 third instar larvae, as indicated; values were normalized relative to RpL32 (internal control), and are shown as mean ± SEM relative to wt (set to 1); * = p<0.05, ** = p<0.01.

DOI: http://dx.doi.org/10.7554/eLife.20882.005

Figure 2—figure supplement 1. Additional analysis of lgs^2-8 during fly development.

(A, B) Leg discs as in main Figure 2 but giving rise to first or second leg, showing similar derepression of dpp. LacZ in the ventral compartments (arrow) of lgs^2-8/lgs^2-8 homozygous larvae as seen in their third leg discs; space bar, 50 mm. (C) Stereo images of eyes from wt or heterozygous mutant females also expressing activated Armadillo (F76) (Freeman and Bienz, 2001), as indicated in the panels, showing comparable suppression of the rough eye phenotype by lgs^2-8/+ as by heterozygosity of the strongest known lgs allele (lgs^20F; Kramps et al., 2002), or by a pygo null allele (pygo^S123; Thompson et al., 2002).

DOI: http://dx.doi.org/10.7554/eLife.20882.006