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. 2017 Jan 6;8(5):7248–7264. doi: 10.18632/oncotarget.14540

Figure 4. Generation of recombinant B95-8/npcEBNA1.

Figure 4

A. Schematic map of bacmids containing EBV genomes for M81, B95-8, or B95-8/npcEBNA1. The EBNA1 DBD (aa 454-608) from NPC genome are highlighted in red. B. BamHI restriction digest of bacmid genomes for B95-8, M81, or B95-8/npcEBNA1. Ethidium bromide stain agarose gel with 1kb ladder markers (left) was subject to Southern blot hybridization with probe for EBV terminal repeat (TR) (middle panel) or IR1/W repeats (right panel). C. Western blot of two independent (a or b) bacmid-transduced 293T producer cell lines for expression of EBNA1 and Actin.