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. 2016 Dec 24;8(5):8189–8205. doi: 10.18632/oncotarget.14138

Figure 3. The downregulation of 45A ncRNA modulates the expression of specific cell cycle related genes.

Figure 3

A. Expression profile of genes involved in cell cycle progression and in cell cycle control in HEK-293-Anti45A/HEK-293-Mock cells; these genes have been considered arbitrarily imposing a cut-off (+35<up-regulated genes<+75.27 and -55.18<down-regulated genes<-1). B. 1-4: Representative GTSE1 IF on SKNBE2-Mock (CTR) (upper panels) and SKNBE2-Anti45A (lower panels). C. High magnification of single cell stained with Red-anti-GTSE1, Blue-Dapi and Green-GFP. D. GTSE1 Western blotting analysis of SKNBE2-Mock and SKNBE2-Anti45A cells. Equal loading of proteins was ensured by β-actin expression. Cropped gel retains important bands of GSTE1 and β-actin.