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. 2016 Dec 26;8(5):8342–8355. doi: 10.18632/oncotarget.14184

Figure 7. Downregulation of NKIRAS2 by miR-1908-5p is implicated in the oncogenic functions of SRSF3.

Figure 7

A. To check the effect of miR-1908-5p/NKIRAS2 on the anchorage-independent proliferation, U2OS cells were transfected with pre-miR-1908-5p or NKIRAS2-specific siRNA for 24 h. Equal numbers of transfected cells were plated in attachment or ultra-low attachment plates. After 48 h post-incubation, cellular viability was calculated by the MTS assay. B-D. U2OS cells were transfected with control (CTRL) or NKIRAS2-specific siRNA for 48 h. Cellular proliferation was calculated by counting viable cells every 24 h (B). Invasive and migratory abilities were assessed by Matrigel invasion (C) and wound healing assays (D), respectively. All experiments are performed more than three times and data represent the mean ± S.D. Asterisk (*) indicates statistical significance of p<0.05 as determined by Student's t-test.