Figure 3. Pluripotency of reprogrammed colonies obtained from SP α6+/c-Kit+ cells.

a. Alkaline phosphatase activity in colonies with an ESC-like morphology analyzed by immunocytochemistry. b. Sox2 and c. Nanog expression in ESC-like colonies by immunofluorescence. Dapi (blue) nuclear staining. d. Cells from the reprogrammed colonies co-express Oct3/4 and Sox2 as shown by flow cytometry analysis. e. Teratomas were generated in testis of NSG mice after transplantation of ESCs as a control (left) or with iPSCs from SP α6⁺/c-Kit⁺ reprogrammed colonies (right). f. Representative images of sections of teratoma obtained from iPSCs and stained with haematoxylin and eosin showing tissues derived from the three germ layers.