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. 2016 Dec 31;8(6):10287–10297. doi: 10.18632/oncotarget.14396

Figure 3. The miR-215-5p inhibits PCDH9 expression by targeting its promoter and 3′ UTR.

Figure 3

A. WB results showing PCDH9 protein levels after over-expression with miR-215-5p mimic or knocking-down with anti-miR-215 inhibitor at the concentration of 5, 10, 20, 50 and100 nM for 48h in U251. The qRT-PCR results showing PCDH9 mRNA levels after over-expression or knocking-down of miR-215-5p in U251 B. n=3 and U87 C. n=3 cell lines. Representative WB results D. and its quantification E. showing PCDH9 protein levels after over-expression or knocking-down of miR-215-5p in U251 and U87 cell lines. Luciferase reporter assays showing the effects of over-expression or knocking-down of miR-215-5p on the wild-type or mutated promoter F. and 3′UTR G. of PCDH9, respectively. H. U251 cells were transfected with wild-type, promoter-mutated (5′ MUT), 3′UTR-mutated (3′MUT) or double mutated PCDH9-HA vector and miR-215-5p mimic. WB results showing PCDH9-HA protein levels detected by HA antibody. Data were presented as means ± s.e.m. For all, ***P<0.001.