Figure 2. The effect of andrographolide on cancer stemness marker expression.

(A)The ALDH1 activity and (B) CD44 positivity of OCSCs treated with or without andrographolide was assessed by flow cytometry and data was presented as percentage of control; (B) mRNA and (C) protein expressions of the stemness markers (Oct4, Nanog, and Sox2) in andrographolide-treated OCSCs. Experiments were performed in triplicate. Values are expressed as mean ± SD. *p < .05 compared to control.