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. 2016 Dec 10;8(3):5092–5110. doi: 10.18632/oncotarget.13867

Figure 4. CUG2 silence inhibits TGF-β signaling, leading to suppression of the EMT.

Figure 4

A. At 48 h post-treatment with CUG siRNA (500 nM), expression of CUG2, E-cadherin, N-cadherin, vimentin, phospho-Smad2, Smad2/3, Snail and Twist in A549-CUG2 and BEAS-CUG2 cells was detected by immunoblotting. (siCon; control siRNA, siCUG2; CUG2 siRNA). B. Cell migration was measured by a wound healing assay in A549- CUG2 and BEAS-CUG2 cells at 48 h post-treatment with CUG2 siRNA. The wound closure areas were monitored by phase-contrast microscopy at a magnification of 100×. The assays were repeated twice. C. An invasion assay was performed with A549-CUG2 and BEAS-CUG2 at 48 h post-treatment with NPM1 siRNA. Scale bar indicates 100 μm. The assays were repeated twice. Each assay was performed in triplicate and error bars indicate SD (***;p< 0.001).