Figure 6. Effect of FLT3 TKI on progression of BaF3 D835Y cells engrafted in syngeneic BALB/c mice.

BaF3 D835Y cells (2 × 10⁶) transfected with a luciferase construct were transplanted into three cohorts of BALB/c mice via tail vein injection with 5 mice per group. On day 5 following transplantation, the level of engraftment was assessed by imaging mice for bioluminescence on an IVIS Spectrum imager. Starting on day 5, mice were then treated twice daily by vehicle, subcutaneous lestaurtinib (20 mg/kg) or once daily sorafenib (10 mg/kg) by oral gavage until day 9, at which point mice were again imaged. This experiment was repeated three times.