Table 1. Chrysotile treatment induces aneuploidy in cultured cell lines.
| Cell lines | Treatment | Number of cells analyzed | Number (%) of aneuploid cells for Chr 8 only | Number (%) of aneuploid cells for Chr 12 only | Number (%) of aneuploid cells for Chr 8 and 12 | Total (%) |
|---|---|---|---|---|---|---|
| HBEC | Untreated | 850 | 0 (0.00) | 0 (0.00) | 0 (0.00) | 0 (0.00) |
| Chrysotile A (2 μg/cm2) | 1661 | 19 (1.14)* | 25 (1.51)* | 4 (0.24) | 48 (2.89)* | |
| MeT5A | Untreated | 683 | 2 (0.29) | 4 (0.59) | 2 (0.29) | 8 (1.17) |
| Chrysotile A (2 μg/cm2) | 815 | 9 (1.10) | 21 (2.58)* | 7 (0.86) | 37 (4.54)* |
Frequencies of aneuploid daughter cells were detected by FISH using centromeric probes specific to human chromosomes 8 and 12 following long-term live-cell imaging. Data are summarized from at least two independent experiments.
*p < 0.001, 2 × 2 χ2 test, compared with frequencies of aneuploidy in untreated cells.