Figure 4. Otub1-stabilized MDMX promotes p53 phosphorylation at S46 and induces apoptosis.
(A) Otub1 expression induces p53-S46P. T-Rex-U2OS-Flag-Otub1 cells treated without or with 2 ug/ml Dox for 24 hours were assayed by IB using indicated antibodies. (B) Otub1-induced MDMX participates in the induction of p53S46P. T-Rex-U2OS-Flag-Otub1 cells transfected with scrambled control or MDMX siRNA followed by treatment of cells without or with 2 ug/ml Dox for 24 hours. The cells were assayed by IB using the indicated antibodies.