Figure 7.

3D reconstruction of Oxt-positive preoptic neurons that innervate the Tel and other brain regions, but not the pituitary, at 6 dpf. A–E″, Skeletonized neurons, shown within the context of the Oxt IHC (A–E; dorsal and lateral views), and shown individually with a color code of the distance along each branch from the soma center (A′–E′; dorsal, lateral, and frontal views). Distance color codes are scaled to the maximum distance of each cell. Note that these cells feature extensive branching throughout the brain, with fibers reaching rostrally into the Tel (A–E′), caudoventrally into the hypothalamus (A–C′, E–E′), caudodorsally into the TeO (C–D′), and several of the branches crossing to the contralateral hemisphere (A–A′, C–E′). F, Registration of these encephalotropic cells shows the contribution of each cell to the brainwide innervation pattern (dorsal, lateral, and frontal views).