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. Author manuscript; available in PMC: 2017 Mar 17.
Published in final edited form as: Biochemistry. 2016 Aug 23;55(35):4971–4981. doi: 10.1021/acs.biochem.6b00347

Table 3.

List of Unique Disulfide Mutants and Disulfide States upon Lipidationa

apoA-I disulfide tryptic fragment [(m/z)charge+] lipid-free locked lipid-bound locked
D13C/V67C T3–T9 [(790.14)4+] no yes
E34C/S55C T5–T7 [(751.34)4+] yes yes
F104C/H162C T14–T26 [(820.05)3+] yes yes
L200C/L233C T32–T35 [(645.44)4+] yes yes
a

Trypsin digest and MS analysis of mutant apoA-I proteins in the lipid-free or lipid-bound conformation yielded the set of ions listed above. Proteins were examined for the presence of the disulfide tryptic fragment in the lipid-free state or after formation of rHDL.