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. 2016 Sep 10;7(42):68292–68302. doi: 10.18632/oncotarget.11944

Figure 2. Cyclic stretch induced the activation and release of IL-1β in HPDLCs.

Figure 2

(A) Real-time PCR result revealed that the expression of IL-1β mRNA increased in response to 6 h cyclic stretch and was reduced after 24 h cyclic stretch. (B) Western blot analyses showed that 6 h cyclic stretch increased the expressions of pro-IL-1β and mature IL-1β proteins. GAPDH was used as a loading control. (C) ELISA assay verified the appearance of IL-1β in the cell-culture medium of HPDLCs in response to 1, 2, 4, 6, 12 and 24 h cyclic stretches and showed that both 4 and 6 h stretches stimulated the release of IL-1β. The appearance of IL-1β in the cell-culture medium of HPDLCs reached a peak after 6 h stretch and then decreased to the control level after 12 h stretch. The results were quantified from at least 3 independent experiments and expressed as mean ± S.E. Statistical significance was calculated using one-way ANOVA with multiple comparisons. *P < 0.05 versus control group; #P< 0.05 versus 6 h group.