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. 2016 Oct 4;7(51):84748–84757. doi: 10.18632/oncotarget.12454

Figure 3. VEGFR2 activation mediates gremlin-induced cytoprotection against UV.

Figure 3

Primary skin keratinocytes were treated with gremlin (25 ng/mL) or with applied VEGFR2 inhibitors (1 μM of SU5416, 100 nM of Axitinib or 5 μM of ZD6474) for 30 min, expression of listed proteins was tested by Western blot assay A. Cells were also subjected to UV radiation, and were cultured in complete medium for applied time, cell viability (B. MTT assay) and cell apoptosis (C. Histone DNA ELISA assay) were tested. Primary skin keratinocytes expressing the scramble control shRNA (“shSCR”) or different VEGFR2 shRNA (“-1/-2”) were treated with gremlin (25 ng/mL) for 30 min, cells were then subjected to UV radiation and cultured for applied time, indicated protein expression D. cell viability E. and apoptosis F. were tested. “Ctrl” stands for un-transfected cells (D). #P < 0.05 vs. gremlin group (B and C). #P < 0.05 vs. gremlin of “shSCR” group (E and F).