Figure 5. Co-exposure to TPL and araC or ADM leads to increased expression of γH2A.X, a marker for DNA double-strand break.

NALM-6/R cells were exposed to araC (5 μM, left panel) or ADM (0.5 μM, right panel) ± TPL (IC₂₀: 10 nM) for 48 h, after which cells were stained with anti-γH2A.X antibody for 1 h, followed by FITC-conjugated secondary antibody for 30 min, and then subjected to flow cytometric analysis.