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. 2016 Aug 8;8(2):2197–2208. doi: 10.18632/oncotarget.11116

Figure 5. USP19 is required for in NHEJ-mediated DNA repair.

Figure 5

A. EJ5-GFP U2OS cells were cotransfected with control or USP19 siRNA and HA-I-SceI. Cells were collected and analyzed for the proportion of GFP-positive cells by flow cytometric. The NHEJ efficiency presented as relative quantification compared with control siRNA. Data are shown as mean ± S.D. *p<0.05, **p<0.01. B. DR-GFP U2OS cells were cotransfected with control or USP19 siRNA and HA-I-SceI. Cells were collected and analyzed for the proportion of GFP-positive cells by flow cytometric. The HR efficiency presented as relative quantification compared with control siRNA. Data are shown as mean ± S.D. C. EJ5-GFP U2OS cells were cotransfected with HA-I-SceI and siRNAs as indicated. Protein levels of HDAC1, HDAC2, USP19 and HA-I-SceI were assessed by Immunoblot. D. EJ5-GFP U2OS cells were cotransfected with HA-I-SceI and siRNAs as in C. Cells were collected and analyzed for the proportion of GFP-positive cells by flow cytometric. The NHEJ efficiency presented as relative quantification compared with control siRNA. Data are shown as mean ± S.D. *p<0.05, **p<0.01.