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. 2016 Nov 25;8(2):3132–3143. doi: 10.18632/oncotarget.13624

Figure 4. Knockout of CD147 by CRISPR/Cas9 resembles the effect of CD147 immunodepletion.

Figure 4

A. MTS cell viability assay showing the effect of CRISPR/Cas9-mediated CD147 knockout on GC-2 cell viability. GC-2-Cas9 cells transfected with gRNA1/2 displayed reduced cell viability after 48 h, compared with the negative control (NC). B. Representative images of western blot analysis of the canonical NFsB factors (p105, p50 and p65), the noncanonical NFκB factors (p100 and p52) and apoptosis markers (cleaved caspase 3) in gRNA1/2 treasfected GC-2-Cas9 cells. β-actin was used as the loading control. C. The corresponding statistical analysis for the western blot (*, p<0.05 vs IgG control), the experiments were repeated 3 times. Values represent the mean±SEM.