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A. Cell viability of SK-Hep1 and MHCC-97H cells when RNF2 was downregulated. B. Cell viability of SMCC7721 and Huh cells when RNF2 was overexpressed. C and D. Colony formation of HCC cells with WT-RNF2 or RNF2-H69Y mutant, or HCC cells with si-RNF2 treatment. The data are expressed as the mean ± SD. The results are representative of three independent experiments. *P < 0.05, **P < 0.01.
