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. 2016 Nov 29;8(2):3144–3155. doi: 10.18632/oncotarget.13673

Figure 4. RNF2 knockdown suppresses invasion and metastasis of HCC cells.

Figure 4

HCC cell lines infected with RNF2 expression vector or its shRNA or control were used in the following studies. A. The wound-healing assay was used to explore the effect of RNF2 on the motility of HCC cells. The percentage of wound healing is shown in the diagrams (left panel). B. Effects of RNF2 knockdown or over-expression on HCC cell invasion in indicated cells in vitro using transwell assay. The diagrams were shown (left panel). C. Representative immunofluorescence (IF) images indicated that RNF2 has an effect on the expression of EMT genes in HCC cells. D. Effects of RNF2 over-expression on tumor metastasis of indicated cells in nude mice (n = 10 per group): the number of metastatic nodules in the lung (left-panel); representative morphological observation of lung metastases (right-upper panel); and histopathological observation of lung sections (right-lower panel). The results are presented as are means ± SD (n = 3 for each panel). Statistical significance was concluded at *P < 0.05, **P < 0.01, ***P < 0.001; # represents no statistical significance.