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. 2016 Dec 10;8(2):3259–3273. doi: 10.18632/oncotarget.13887

Figure 5. LEF1-dependent expression of muscle differentiation markers in RMS cell lines.

Figure 5

(A) Expression of MYOD, MYOGENIN, MYH1, DESMIN and CKM in Rh41 LEF1 KD, RMS-13 LEF1 KD and TE671 LEF1 KD are shown as fold expression to the respective control cells that were set to 1 (dashed line). (B) Expression of the same markers in the same cells after transfection with scrambled siRNA (scr siRNA) or CTNNB1 siRNA. Significances are shown for values after transfection with scr siRNA versus CTNNB1 siRNA. (A and B) Gene expression levels were normalized to 18S rRNA expression levels. Data represent mean + SEM of at least two independent experiments performed in duplicates and measured in triplicates; *P< 0.05, ***P < 0.001 by Students t-test.