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. 1990 Mar;87(5):1884–1888. doi: 10.1073/pnas.87.5.1884

Cytoskeletal reorganizations responsible for the phorbol ester-induced formation of cytoplasmic processes: possible involvement of intermediate filaments.

A D Bershadsky 1, O Y Ivanova 1, L A Lyass 1, O Y Pletyushkina 1, J M Vasiliev 1, I M Gelfand 1
PMCID: PMC53588  PMID: 1968640

Abstract

The tumor promoter phorbol 12-myristate 13-acetate (PMA) induces characteristic reversible changes of cell shape in certain fibroblastic lines: motile lamellas are transformed into noncontractile narrow processes; simultaneously, the actin microfilament network of lamellas is locally disorganized. This reaction to PMA may be regarded as a prototype of reorganizations involving formation of stable cytoplasmic processes. Specific drugs, Taxol and Colcemid, were used to study the role of microtubules and vimentin-containing intermediate filaments (IF) in the development of PMA-induced reorganizations. PMA readily induced formation of noncontractile processes in Taxol-treated fibroblasts; these cells had a profoundly altered microtubular system but noncollapsed IF. A short (1 hr) exposure to PMA induced formation of processes in control cells but not in the Colcemid-treated cells, which had depolymerized microtubules and IF that collapsed around the nucleus. Longer (3-4 hr) exposure of the Colcemid-treated cells to PMA induced partial reversal of the IF collapse; those parts of the peripheral lamellas that contained IF were transformed into narrow noncontractile processes. It is suggested that the local interaction of IF with the actin system is an essential step in the formation of processes from lamellas. The microtubular system controls distribution of IF in the cytoplasm and thus plays an indirect role in the reorganization of the actin cortex.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akiyama T., Nishida E., Ishida J., Saji N., Ogawara H., Hoshi M., Miyata Y., Sakai H. Purified protein kinase C phosphorylates microtubule-associated protein 2. J Biol Chem. 1986 Nov 25;261(33):15648–15651. [PubMed] [Google Scholar]
  2. Bershadskii A. D., Brodskaia R. M., Mansurov P. G., Stavrovskaia A. A., Stromskaia T. P. Sviaz' mezhdu prikrepleniem kletok k substratu i ikh proliferativnymi kharakteristikami. Eksp Onkol. 1984;6(1):27–32. [PubMed] [Google Scholar]
  3. Bershadsky A. D., Tint I. S., Svitkina T. M. Association of intermediate filaments with vinculin-containing adhesion plaques of fibroblasts. Cell Motil Cytoskeleton. 1987;8(3):274–283. doi: 10.1002/cm.970080308. [DOI] [PubMed] [Google Scholar]
  4. Danowski B. A., Harris A. K. Changes in fibroblast contractility, morphology, and adhesion in response to a phorbol ester tumor promoter. Exp Cell Res. 1988 Jul;177(1):47–59. doi: 10.1016/0014-4827(88)90024-9. [DOI] [PubMed] [Google Scholar]
  5. Dugina V. B., Svitkina T. M., Vasiliev J. M., Gelfand I. M. Special type of morphological reorganization induced by phorbol ester: reversible partition of cell into motile and stable domains. Proc Natl Acad Sci U S A. 1987 Jun;84(12):4122–4125. doi: 10.1073/pnas.84.12.4122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Farber R. A., Liskay R. M. Karyotypic analysis of a near-diploid established mouse cell line. Cytogenet Cell Genet. 1974;13(4):384–396. doi: 10.1159/000130288. [DOI] [PubMed] [Google Scholar]
  7. Forry-Schaudies S., Murray J. M., Toyama Y., Holtzer H. Effects of colcemid and taxol on microtubules and intermediate filaments in chick embryo fibroblasts. Cell Motil Cytoskeleton. 1986;6(3):324–338. doi: 10.1002/cm.970060309. [DOI] [PubMed] [Google Scholar]
  8. Geuens G., de Brabander M., Nuydens R., De Mey J. The interaction between microtubules and intermediate filaments in cultured cells treated with taxol and nocodazole. Cell Biol Int Rep. 1983 Jan;7(1):35–47. doi: 10.1016/0309-1651(83)90103-0. [DOI] [PubMed] [Google Scholar]
  9. Green K. J., Goldman R. D. The effects of taxol on cytoskeletal components in cultured fibroblasts and epithelial cells. Cell Motil. 1983;3(4):283–305. doi: 10.1002/cm.970030402. [DOI] [PubMed] [Google Scholar]
  10. Hirokawa N., Hisanaga S., Shiomura Y. MAP2 is a component of crossbridges between microtubules and neurofilaments in the neuronal cytoskeleton: quick-freeze, deep-etch immunoelectron microscopy and reconstitution studies. J Neurosci. 1988 Aug;8(8):2769–2779. doi: 10.1523/JNEUROSCI.08-08-02769.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hollenbeck P. J., Bershadsky A. D., Pletjushkina O. Y., Tint I. S., Vasiliev J. M. Intermediate filament collapse is an ATP-dependent and actin-dependent process. J Cell Sci. 1989 Apr;92(Pt 4):621–631. doi: 10.1242/jcs.92.4.621. [DOI] [PubMed] [Google Scholar]
  12. Huang C. K., Devanney J. F., Kennedy S. P. Vimentin, a cytoskeletal substrate of protein kinase C. Biochem Biophys Res Commun. 1988 Feb 15;150(3):1006–1011. doi: 10.1016/0006-291x(88)90728-0. [DOI] [PubMed] [Google Scholar]
  13. Lyass L. A., Bershadsky A. D., Vasiliev J. M., Gelfand I. M. Microtubule-dependent effect of phorbol ester on the contractility of cytoskeleton of cultured fibroblasts. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9538–9541. doi: 10.1073/pnas.85.24.9538. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pollard T. D., Selden S. C., Maupin P. Interaction of actin filaments with microtubules. J Cell Biol. 1984 Jul;99(1 Pt 2):33s–37s. doi: 10.1083/jcb.99.1.33s. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Vasiliev J. M. Actin cortex and microtubular system in morphogenesis: cooperation and competition. J Cell Sci Suppl. 1987;8:1–18. doi: 10.1242/jcs.1987.supplement_8.1. [DOI] [PubMed] [Google Scholar]
  16. Vasiliev J. M., Gelfand I. M. Membrane-induced segregation of motile and stable domains in cytoplasm: possible role in morphological differentiation of tissue cells. Cell Differ. 1988 Jul;24(2):75–81. doi: 10.1016/0045-6039(88)90059-0. [DOI] [PubMed] [Google Scholar]

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