Figure 1.

RT-qPCR analysis of chondrogenic gene expression in chondrocytes from MACT (CaReS) grafts. Chondrocytes were isolated from the healthy site of the articular cartilage in patients for 3 different age group subjects (20.5 ± 3.7, 31.8 ± 1.9, 41.9 ± 4.1 years) (n = 8-9 per group) and cultivated in MACT (CaReS) grafts for 14 days. Gene expression of MACT cells at different ages were normalized to their respective GAPDH expression levels. Data expressed as median and range with Tukey box and whisker plot: lower box = 75 percentile, upper box = 25 percentile, whisker = non-outlier range, dot = outlier, asterisk = extreme outlier (>1.5-fold above/below the box).