Article title: “A nonapoptotic role for CASP2/caspase 2: modulation of autophagy”
Authors: Meenakshi Tiwari, Lokendra K. Sharma, Difernando Vanegas, Danielle A. Callaway, Yidong Bai, James D. Lechleiter and Brian Herman
Journal: Autophagy
Bibliometrics: Volume 10, Issue 6, pages 1054–1070
DOI: 10.4161/auto.28528.
It has been noted by the authors that in the Figure 5E, the loading control TUBA (for MAPK8/9) was inadvertently wrongly positioned. The revised corrected image of loading control (TUBA) for the Figure 5E along with the corrected densitometry analysis (Figure 5F) in this Corrigendum. However, revised analysis has not changed the interpretation of the data.
Figure 5.
Role of MAPKs in CASP2-mediated modulation of autophagy. (A to F) Protein lysates were prepared from different batches of WT and casp2−/− MEFs (loaded in separate lanes 1 to 3 or 1 and 2 for MAPK8/9), cultured in complete medium in the absence of stressors. Western blotting was performed to detect the levels of active (phosphorylated) and total (non-phosphorylated) MAPKs using specific antibodies against (A) p-MAPK1/3, MAPK1/3 (C) p-MAPK14, MAPK14 and (E) p-MAPK8/9, and MAPK8/9 as described in the Materials and methods. The same blots were reprobed for TUBA that served as a loading control. Shown are the representative blots. (B, D, F) Densitometric analysis was performed using ImageJ to detect the expression levels. Y-axis represents expression levels of active versus total MAPK as percent of the loading control. Error bar represents ±SEM. Statistical significance was determined by the Student t test, comparing WT versus casp2−/−. **, P ≤0.01, *, P ≤0.05, NS, P ≥ 0.05, experiments repeated at least 3 times. (G) Effects of MAPK inhibitors: SP600125 (10 µM), SB203580 (10 µM), and U0126 (20 µM) on CASP2-mediated autophagy modulation. WT and casp2−/− MEFs were incubated with these inhibitors for 24 h followed by PepA (1 µM) and EST (10 µM) treatment for 6 h to determine autophagy flux. Autophagy was detected by western blotting to determine the levels of LC3 (LC3-I and LC3-II). GAPDH was used as a loading control. Shown is a representative blot, the experiment was repeated 3 times.
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