Figure 2.

Overexpression of Cbl-b inhibited MDR cancer cell migration in vitro and in vivo. (A) H&E staining P-gp or Cbl-b of adenocarcinoma tissues of gastric cancer patients was shown (20×). (B) Wound closure was monitored at 24 hours after the cells were transfected with Cbl-b WT and empty vector plasmid for 48 hours (upper). The wound closure was further quantified (lower). *P < .05. (C) The cells on Transwell membranes were monitored at 24 hours after Cbl-b WT transfection for 48 hours (upper). The migration ability was further quantified (lower). *P < .05. (D) The treated SGC7901/Adr cells were injected into mice and observed by the [¹⁸F] FDG luciferase signals. The images were obtained using micro-PET at 21 days after cell injection. (E) H&E staining of metastatic tumor colonies in the lungs were shown (40×). The numbers of metastatic tumor colonies in the lungs of nude mice injected with Cbl-b WT-transfected cells were monitored. Compared with those injected with the control cells, *P < .05.