(
A) Epididymal sperm count (mean ± SEM) from littermates at ages 2–3 months.
CatSperz het (
+/-, blue; 2.4 ± 0.1) versus null (
-/-, green; 2.4 ± 0.2) cells (10
7). (
B) Average litter size from all males in the mating test, grouped by male and female genotype. (
C) IVF rate calculated by counting fertilized eggs (2 cell stage) 20 and 27–30 hr after coitus. Data are expressed as a scatter plot of mean percentage from
CatSperz+/- (20 hr, 70 ± 15; 27–30 hr, 94.4 ± 2.5) and
CatSperz-/- (20 hr, 21.3 ± 7.6; 27–30 hr, 24.6 ± 7.4). **p=0.0097 (One-way ANOVA and F test). See also
Figure 2D. (
D) Sperm motility parameters measured by computer assisted sperm analysis (CASA) from
CatSperz het (
+/-) versus null (
-/-) male mice. 5 min (basal, gray) and 90 min (capacitated, black) after incubation in HTF. ALH of
CatSperz+/- (basal, 15.5 ± 0.4; 120 min, 17.3 ± 0.4, p=0.0002). Data are mean ± SEM. N = 4. (
E) Capacitation-associated protein tyrosine phosphorylation of
CatSperz -/- spermatozoa.