Video 1. Movement of free swimming CatSperz +/- and -/- spermatozoa; related to Figure 2.
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DOI: 10.7554/eLife.23082.014
Uncapacitated (left) and 90 min capacitated (right) spermatozoa were allowed to disperse for 10 min pre-incubation in a 37°C chamber containing HEPES-HTF; free swimming sperm cells recorded within the next 5 min; video rate 20 fps (1/5 speed), 1 s movies; head trace to track swimming trajectory. (A) CatSperz+/- and (B) CatSperz-/- spermatozoa.